MiRNA155-DNA甲基化介导mitoKATP调节线粒体自噬在芪参益气滴丸改善心肌缺血再灌注损伤中的分子机制理论探讨

A theoretical study of mi RNA155-DNA methylation mediated mitok ATP regulating mitochondrial autophagy in the improvement of myocardial ischemia-reperfusion injury by Qishen Yiqi Dropping Pills

在急性心肌缺血状态下,miRNA表达可以调控相关基因及蛋白,减少心肌细胞损伤,进而发挥保护心肌的作用,然而具体机制仍需进一步探究。近期研究发现,mitoKATP通道的开放可以调节线粒体自噬,且miRNA-DNA甲基化的启动在诱导细胞自噬发生方面发挥了调控作用。笔者团队前期研究发现,中药复方芪参益气滴丸通过介导mitoKATP通道调节线粒体自噬,进而明显改善心肌缺血;且动物实验证实,miR-155在细胞自噬调节、炎症反应、血管平滑肌细胞迁移方面有显著作用。故笔者提出假设:芪参益气滴丸可以激活miRNA155-DNA甲基化介导的mitoKATP开放,进而调节线粒体自噬,改善心肌缺血。本文通过阐述线粒体自噬与心肌缺血后的氧化应激损伤的关联性,探讨芪参益气滴丸通过调控MiRNA155-DNA甲基化介导mitoKATP调节线粒体自噬,进而改善心肌缺血再灌注损伤的可能机制,为相关研究提供理论思路。

In the state of acute myocardial ischemia,miRNA expression can regulate related genes and proteins,reduce myocardial cell damage,and thus play a protective role in the myocardium. However,the specific mechanism still needs to be further explored. Recent studies have found that the opening of the mitoKATP channel can regulate mitochondrial autophagy,and the initiation of miRNA-DNA methylation plays a regulatory role in inducing cell autophagy. The applicant research team previously found that Qishen Yiqi Dropping Pills could significantly improve myocardial ischemia by mediating MitokATP channels to regulate mitochondrial autophagy,and animal experiments confirmed that miR-155 played a significant role in the aspect of autophagy regulates,inflammatory reaction and vascular smooth muscle cell migration. Therefore,the applicant innovatively proposed that Qishen Yiqi Dropping Pills can regulate miRNA155-DNA methylation to mediate the opening of mitoKATP,thereby regulating mitochondrial autophagy and improving myocardial ischemia. In this paper,the association between mitochondrial autophagy and oxidative stress injury after myocardial ischemia was described,and the possible mechanism of Qisen Yiqi Dropping Pills regulating mitochondrial autophagy by regulating miRNA155-DNA methylation to mediate MitokATP to improve myocardial ischemia reperfusion injury was discussed,so as to provide theoretical ideas for related research.