UHPLC-Q-Orbitrap-MS/MS法分析5种紫金牛属植物的黄酮类成分

Characterization of Flavonoids from Five Species of Ardisia Using UHPLC-Q-Orbitrap-MS/MS

目的分析5种紫金牛属药用植物(细罗伞、九管血、朱砂根、月月红和雪下红)的黄酮类成分。方法采用超高效液相色谱(UHPLC)技术分离5种紫金牛属植物的黄酮类成分,在高分辨质谱仪(Q-Orbitrap-MS/MS)正、负离子模式下采集碎片离子信息,应用CD2.1软件(Thermo Fisher)完成数据初步整理后进行数据库(mzCloud,mzVault,ChemSpider)检索比对。结果从5种紫金牛属植物中共鉴定出38种黄酮类成分,细罗伞、九管血、朱砂根、月月红和雪下红分别鉴定出37、34、29、36和30种黄酮类化合物,有26种黄酮类成分为5种紫金牛属植物的共有成分。结论该鉴定方法精确、可靠、高效,能快速鉴定出紫金牛属植物的黄酮类成分,为紫金牛属药用植物的药效物质基础和开发利用研究提供科学依据。

Objective To characterize flavonoids of Ardisia affinis, Ardisia brevicaulis, Ardisia crenata, Ardisia faberi, and Ardisia villosa and provide a scientific basis for development and utilization of the Ardisia genus.Methods The flavonoids were separated by Ultra-high performance liquid chromatography(UHPLC),and the ion information of the fragments was further collected under positive and negative ion modes by a high-resolution mass spectrometer(Q-Orbitrap-MS/MS). The compounds processed by CD2.1(Thermo Fisher) were characterized by matching with the databases(mzCloud, mzVault, and ChemSpider). Results A total of 38 compounds were characterized in Ardisia affinis(37),Ardisia brevicaulis(34),Ardisia crenata(29),Ardisia faberi(36),and Ardisia villosa(30), and there were 26 common flavonoids in the five species of Ardisia. Conclusion The method established in the current study was accurate, reliable, and effective for characterization of the flavonoids of Ardisia,which can provide a scientific basis for the development and utilization of medicinal resources of the Ardisia.