固本化湿降脂汤调节脾虚湿阻型单纯性肥胖大鼠代谢异常的相关性研究

Study on the Relevance of Guben Huashi Jiangzhi Decoction to Regulate Metabolic Abnormalities in Simple Obesity Rats with Spleen Deficiency and Dampness Resistance

目的探讨固本化湿降脂汤对脾虚湿阻型单纯性肥胖大鼠代谢异常的调节作用。方法将48只SD大鼠随机分为正常组、模型组、奥利司他阳性组及固本化湿降脂汤高、中、低(34.4、17.2、8.6 g·kg^(-1))剂量组,每组8只。通过高脂饮食喂养10周,同时结合冰糖水灌胃16 d,游泳3周,建立脾虚湿阻型单纯性肥胖大鼠模型。模型复制成功后,对应灌胃给药,并观察大鼠外观表征、舌象、远红外热成像的变化;采用全自动生化分析仪检测血清甘油三酯(TG)、高密度脂蛋白(HDL-C)、低密度脂蛋白(LDL-C)、总胆固醇(TC)含量;采用ELISA法检测血清尿素氮(BUN)、尿酸(UA)、胃动素(MTL)、活性肠肽(VIP)水平;采用定磷法检测心肌细胞膜Ca^(2+)-ATP酶、Na^(+)-K^(+)-ATP酶活力。结果与模型组比较,固本化湿降脂汤组大鼠血清TG、TC、LDL-C含量表达不同程度降低(P<0.01),HDL-C含量表达升高(P<0.05);BUN、UA、VIP含量表达不同程度降低(P<0.01,P<0.05),MTL含量表达升高(P<0.01);心肌细胞膜Na^(+)-K^(+)-ATP酶(P<0.01)、Ca^(2+)-ATP酶活力升高(P<0.05)。结论固本化湿降脂汤可以调节脾虚湿阻型单纯性肥胖大鼠代谢异常,发挥对脾虚湿阻型单纯性肥胖大鼠的治疗作用。

Objective To investigate the regulation of Guben Huashi Jiangzhi decoction on metabolic abnormalities in simple obesity rats with spleen deficiency and dampness resistance.Methods Forty-eight Sprague-Dawley(SD)rats were randomly divided into six group with each group of eight.Simple obesity rats model with spleen deficiency and dampness resistance was established by feeding high-fat diet for ten weeks,and at the same time by gavage of crystal sugar water sixteen days,and forced swimming for three weeks.After the model was copied successfally,the drug was administered by gavage.The changes in appearance phenotype,tongue image,and far-infrared thermography in rats were observed.Serum triglyceride(TG),high-density lipoprotein(HDL-C),low-density lipoprotein(LDL-C),and total cholesterol(TC)levels were measured using a fully automated biochemical analyzer.Urea nitrogen(BUN),uric acid(UA),motilin(MTL)and vasoactive intestinal peptide(VIP)levels in serum were determined by ELISA.The activities of Ca^(2+)-ATPase and Na^(+)-K^(+)-ATPase in cardiomyocytes were measured by phosphorus fixation.Results Compared with the model group,the contents of TG,TC and LDL-C were decreased to different degrees(P<0.01),the expression of HDL-C was increased(P<0.05);the contents of BUN,UA,and VIP were decreased to different degrees(P<0.01,P<0.05),the expression of MTL was increased(P<0.01),and the activities of Na;-K;-ATPase(P<0.01)and Ca^(2+)-ATPase in myocardial cell membrane were increased(P<0.05)in Guben Huashi Jiangzhi decoction group.Conclusion Guben Huashi Jiangzhi decoction can regulate metabolic abnormalities in simple obesity rats with spleen deficiency and dampness resistance,further exert therapeutic effects.