炎症因子TNF-α对人体皮肤细胞的影响以及有效提取物的筛选

Effect of inflammatory factor TNF-α on human skin cells and screening of effective extracts

本文首先以炎症因子TNF-α为刺激物作用于人体皮肤细胞,探究炎症因子对人体皮肤的影响。结果表明炎症因子TNF-α使得皮肤细胞内胶原蛋白含量表达异常,打破氧化还原系统影响人体皮肤,具有一定的加快皮肤老化作用,同时TNF-α可以作为刺激物建立慢性炎性皮肤衰老模型。后续实验通过TNF-α建立慢性炎性衰老模型,分别考察与比较维生素C、龙血竭提取物、铁皮石斛提取物对炎性衰老的抑制作用。结果表明,维生素C作为公认的抗衰老物质同样具有抑制炎性衰老功效;其次龙血竭提取物、铁皮石斛提取物同样具有一定的抑制炎性衰老功效。

Papain is a natural sulfhydryl protease of removing aging skin and eliminating melanin, which has skin whitening and skin rejuvenation effects.This enzyme needs to be stored at appropriate temperature, humidity and pH, otherwise it will easy lose its activity during cosmetics processing because of improper operation. Compared with freeIn order to explore the influence of inflammatory factors on human skin, inflammatory factor TNF-α was used as a stimulant to act on human skin cells. In this study, TNF-α was used as a stimulant to stimulate human skin cells. The results show that the content of senescence related secretion phenotype, IL-6 and IL-8, in the cells increases, and the cells are under the senescence state. The contents of type I collagen per protein in human skin fibroblasts, which are treated with 20, 100 and 200 ng/mL TNF-α for 24 h, are 2.740, 2.270 and 1.595 mg, respectively, lower than those in the control group;after 4 h of treatment, the contents of MMP-1 per protein are 3.10, 3.12 and 3.10 ng, respectively, higher than those in the control group. At the same time, compared with the control group, the content of intracellular reactive oxygen species is increased, and the balance of intracellular redox system is broken after the keratinocytes exposed to TNF-α. From the above experimental results, the inflammatory factor TNF-α causes the expression of collagen in skin cells abnormal, breaks the redox system, and has a certain effect on accelerating skin aging. Therefore, TNF-α can be used as a stimulant to establish a chronic inflammatory skin aging model. Follow-up experiments established a chronic inflammatory aging model through 200 ng/mL TNF-α, and investigated and compared the inhibitory effects of vitamin C, dragon’s blood extract, and Dendrobium officinale extract on it. The results show that, compared with the control group, 0.1 mg/mL vitamin C, 0.06 mg/mL dragon’s blood, and 0.5 mg/mL Dendrobium officinale extract can effectively promote the synthesis of intracellular collagen, and the contents per unit protein are 4.95, 4.84, 4.08 mg, respectively;can inhibit the expression of MMP-1, and its contents per unit protein are 1.31, 0.84, and 1.26 ng, respectively;can clear the content of reactive oxygen species in the cells and maintain the intracellular redox balance. The results show that vitamin C, as a recognized anti-aging substance, has the same anti-inflammatory aging effect;the extract of Resina draconis and Dendrobium officinale also have certain anti-inflammatory aging effect.