宏基因组二代测序在结核性脑膜炎早期诊断中的应用

Application of Metagenomic Next-generation Sequencing in Early Diagnosis of Tuberculous Meningitis

结核性脑膜炎(TBM)是一种高致死率中枢神经系统感染性疾病,其早期诊断面临着重大挑战。宏基因组二代测序(mNGS)作为一种新的测序技术,无需进行培养,可直接对样本中微生物DNA或RNA进行快速的检测,近年来在感染领域备受关注。目前,研究报道mNGS在特殊病原体的检测方面具有优势,特别是常规诊断方法存在局限性的领域,例如TBM、新发传染性疾病等。本文现就常见的mNGS技术测序原理、mNGS技术在TBM不同人群中的临床使用情况及特点进行综述。

Tuberculous meningitis(TBM)is a central nervous system infectious disease with high mortality,and its early diagnosis is facing major challenges.As a new sequencing technology,metagenomic next-generation sequencing(mNGS)can directly detect microbial DNA or RNA in samples without culture,which has attracted much attention in the field of infection in recent years.At present,studies have reported that mNGS has advantages in the detection of special pathogens,especially in areas where conventional diagnostic methods have limitations,such as TBM and emerging infectious diseases.This paper reviews the common sequencing principle of mNGS technology,and the clinical application and characteristics of mNGS technology in different TBM populations.