脑蛋白水解物-Ⅰ对D-半乳糖致衰老小鼠的抗衰老作用

Anti-aging effect of cerebroprotein hydrolysate-Ⅰ on D-galactose-induced aging mice

目的探讨脑蛋白水解物-Ⅰ(CH-Ⅰ)对D-半乳糖致衰老小鼠的抗衰老作用。方法将32只C57小鼠随机分为对照组、模型组、CH-Ⅰ低剂量组(3 mg/kg)和CH-Ⅰ高剂量组(6 mg/kg),模型组及CH-Ⅰ组小鼠颈背部皮下注射D-半乳糖150 mg/kg,对照组小鼠颈背部皮下注射等量生理盐水,注射3周后,给药组小鼠腹腔注射相应剂量的CH-Ⅰ,对照组及模型组小鼠腹腔注射等量生理盐水,持续给药5周后,进行老化度评分和旷场实验。比色法检测小鼠海马组织中超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-Px)活性和丙二醛(MDA)含量。尼氏染色观察小鼠海马区神经元损伤。结果与对照组相比,模型组小鼠的老化度评分明显增高(P<0.01),自主活动和探索行为减弱,海马组织中SOD、GSH-Px活性明显降低(P<0.01),MDA含量显著增加(P<0.01),海马CA1区神经元显著损伤。经CH-Ⅰ治疗后,可显著降低衰老小鼠的老化度评分(P<0.05,P<0.01),提高小鼠的自主行为能力,提高海马组织中SOD、GSH-Px活性(P<0.05,P<0.01),减少MDA含量(P<0.05,P<0.01),改善海马组织CA1区神经元损伤。结论CH-Ⅰ可以有效改善D-半乳糖模型小鼠的老化程度和自主行为,提高海马组织的抗氧化能力,减少海马神经元损伤。

Objective To investigate the anti-aging effect of cerebroprotein hydrolysate-Ⅰ(CH-Ⅰ)on D-galactose-induced aging mice.Methods A total of 32 C57 mice were randomly divided into the control group,the model group,the CH-Ⅰ low-dose group(3 mg/kg)and the CH-Ⅰhigh-dose group(6 mg/kg).The model group and the two CH-Ⅰ groups were injected with 150 mg/kg D-galactose subcutaneously on the back of the neck.The control group were injected with the same amount of normal saline subcutaneously on the back of the neck.At 3 weeks after injection,the two CH-Ⅰ groups were injected intraperitoneally with the corresponding dose of CH-Ⅰ,and the control group and the model group were intraperitoneally injected with the same amount of normal saline.After continuous administration for5 weeks,the aging degree scoring and open field experiment were performed.Colorimetric method was used to detect superoxide dismutase(SOD),glutathione peroxidase(GSH-Px)activity and malonaldehyde(MDA)content in hippocampal tissue.Nissl staining method was used to observe neuronal damage in hippocampus.Results Compared with the control group,the aging score in the model group was significantly increased(P<0.01),autonomous activities and exploratory behaviors were weakened,SOD and GSH-Px activities in hippocampus tissue were significantly reduced(P<0.01),MDA content was significant increased(P<0.01),and neurons in CA1 area of hippocampus were significantly damaged.CH-Ⅰ treatment significantly reduced aging score of aging mice(P<0.05,P<0.01),improved autonomous activities,increased SOD and GSH-Px activities in hippocampus(P<0.05,P<0.01),reduced MDA content(P<0.05,P<0.01),and improved neuron damage in CA1 area of hippocampus.Conclusion CH-Ⅰ can effectively improve aging score and autonomous activities of D-galactose model mice,improve antioxidant capacity of hippocampal tissue,and reduce neuron damage of hippocampus.