尿液中miRNA-24-3p联合miRNA-222-3p检测在前列腺癌中的诊断及临床意义

Diagnosis and clinical significance of miR-24b-3p combined with miR-222-3p in urinary in prostate cancer

目的:建立尿液miRNA检测板,作为诊断前列腺癌(PC)的无创性生物标志物。方法:采用miRNAs芯片分析对照1组(6名)及PC1组(18例)中miRNAs表达,并进一步利用qRT-PCR对20名对照2组及59例PC2组(GS26组22例,GS27组19例,GS28组18例)尿液、血清标本进行差异基因的进一步验证。应用受试者工作特征(ROC)曲线分析组合诊断模型的准确真实性。结果:获得在PC1组中20个miRNAs差异表达谱,与对照1组相比,miRNA-24-3p及miRNA-222-3p显著表达下调(T=5.79、4.59,均P<0.05),用于PC诊断检测。在扩大样本量的PC患者尿液及血清样本中,根据GS分组的GS26、GS27及GS28组中,与对照2组相比,miRNA-24-3p联合miRNA-222-3p表达水平也都显著下调(t=3.89,P<0.05),ROC分析联合诊断模型具有较高的准确度(曲线下面积为0.93,OR=1.37,95%CI:0.92~1.76,P=0.02)。结论:在尿液、血清样本中建立了miRNA-24-3p联合miRNA-222-3p组合模型,可作为诊断PC的无创性生物标志物。

Objective:To establish a urine miRNA detection panel as a non-invasive biomarker helping diagnosing prostate cancer(PC).Methods:A total of 18 PC1 group patients and 6 normal control group 1 urinary samples were analyzed through a miRNA microarray process,and further using quantitative real-time polymerase chain reaction,differentially expressed miRNAs were detected in urinary and serum of the PC2 patients(n=59),including 22 GS26,19 GS27 and 18 samples of GS28,20 normal samples as the control group 2.The accuracy and authenticity of the combined diagnostic model should be analyzed by the receiver operating characteristic(ROC)curve.Results:The differential expression profiles of 20 miRNAs in PC1 group were identified,including miR-24b-3p and miR-222-3p for PC detection,compared with control group1(T=5.79,4.59,all P<0.05).Moreover,in the urine and serum samples of PC samples with expanded samples,the expression of miR-24b-3p combined with miR-222-3p in GS6,GS7 and GS8 groups grouped according to GS score were significantly decreased than those in the control group 2(t=3.89,P<0.05).The combined diagnostic model of ROC analysis has high accuracy(area under curve=0.93,OR=1.37,95%CI:0.92-1.76,P=0.02).Conclusion:A combined model of miR-24-3p and miR-222-3p in urinary and serum samples are established,which can be acted as a non-invasive biomarker helping diagnosing PC.