牛奶中丙酸杆菌的筛选与鉴定

Screening and Identification of Propionibacterium in Milk

为了研究牛奶中丙酸杆菌的筛选与鉴定方法。本文运用高效液相色谱法对分离纯化后的丙酸杆菌进行定量转化,完成筛选和鉴定方法设计。结果表明,在牛奶制备样品中能筛选出不同形态的丙酸杆菌,且产生丙酸的效果有所增加,利用牛奶作为培养基可促进丙酸的生成;以葡萄糖作为培养基,在240 h内生成的丙酸含量为7.0 g·L^(-1)。

In order to study the screening and identification methods of Propionibacterium in milk,this paper quantitatively transformed the isolated and purified Propionibacterium by high performance liquid chromatography,and completed the design of screening and identification methods.The results showed that different forms of Propionibacterium could be screened in the milk preparation samples,and the effect of producing propionic acid was increased.Using milk as a medium could promote the production of propionic acid;using glucose as a medium within 240 h,the resulting propionic acid content was 7.0 g·L^(-1).