番茄热激因子基因SlHsfC1的表达特性与耐寒功能分析

Analysis of Expression Characteristicsand Cold-Tolerance Function of the Heat Shock Factor C1 Gene in Tomato

番茄热激因子基因SlHsfC1受低温诱导表达,但是否具有耐寒功能尚不清楚.本研究分析了番茄SlHsfC1基因在低温、高温、水淹和盐胁迫下的表达特性,以及该基因过表达对转基因植株耐寒性的影响;初步证实该基因受到低温、高温、水淹和盐胁迫时可诱导表达,过表达能增强转基因番茄植株的耐寒性.该结果为今后揭示该基因参与番茄冷调控的分子机制奠定了基础.

The expression of tomato heat shock factor gene SlHsfC1 can be induced by low temperature,but it is not clear whether it has the function of cold tolerance.Therefore,this study analyzed the expression characteristics of tomato SlHsfC1 gene under low temperature,high temperature,water-logging and salt stress,and the effect of overexpression of this gene on cold tolerance of transgenic plants.It was confirmed that expression of this gene can be induced by low temperature,high temperature,water-logging and salt stress.Overexpression of SlHsfC1 gene can enhance the cold tolerance of transgenic tomato plants.The results laid the foundation for revealing the molecular mechanism of this gene involved in regulation of tomato cold tolerance in the future.