3D电极的介电泳力与惯性力的粒子连续分选仿真

Particle continuous separation simulation based on 3D electrode coupled with dielectric electrophoresis force and inertia force

细胞分选在生物医学中起着重要的作用,而其中介电泳分选由于其无需生物标记,对粒子损伤小等优势得到了广泛的应用。本文设计了一种结合三维(3D)电极的介电泳力和收缩-扩张结构的惯性力的微流控芯片,通过COMSOL Multiphysics仿真软件对流体的流速分布、电场分布及粒子的运动轨迹进行仿真分析。仿真结果表明:三维电极相较于传统的平面电极,能够提供粒子垂直运动方向上的非均匀电场,更有助于实现粒子的高效率分选。此外,当粒子随流体运动时,不同的流道收缩-膨胀比分选效果不同,当收缩-膨胀比为3︰1时,分选效果会更好。通过仿真证明了所设计结构的有效性,确定了芯片尺寸,为后续的粒子连续高通量高效率分选,提供了重要的参考价值。

Cell separation plays an important role in biomedical applications.Dielectrophoresis is widely used due to its advantages of no requirement for biological markers and no damage caused to particles.A microfluidic chip combined with the electrophoretic force of the 3D electrode and the inertia force of the contract-expansion structure is designed.COMSOL Mutiphysics simulation software is used to analyze the flow field,the electrode field and particle trajectory.The simulation results show that compared with traditional planar electrodes,the 3D electrodes can provide non-uniform electric field in vertical direction,which is more useful to realize efficient particle separation.In addition,when the particles move with the fluid,the separation results are different with different contraction-expansion ratio.The sorting effect is better with when the contraction-expansion ratio is 3︰1.The designed structure is proved effective through the simulations,and the size of the micro-device is confirmed.This structure provides an important reference value for continuous high throughput and high efficiency separation of particles.