新疆棉区田间棉铃虫种群对Bt Cry1Ac毒蛋白的敏感性研究

Frequency of Bt resistant alleles in wild cotton bollworm populations in Xinjiang

为了明确新疆棉区棉铃虫Helicoverpa armigera(Hübner)种群对Bt Cry1Ac毒蛋白的敏感性变化,本次研究采用单雌系F;/F;代诊断剂量法于2013-2019年连续监测了新疆库尔勒市棉铃虫种群对Cry1Ac毒蛋白的抗性频率以及种群敏感度的变化。结果表明,2013-2019年新疆库尔勒市棉铃虫种群相对平均发育级别分别为0.328、0.386、0.539、0.572、0.565、0.542和0.562。棉铃虫种群的相对平均发育级别呈逐年升高的趋势,表明棉铃虫对Cry1Ac毒蛋白的敏感度降低,但尚未发现相对发育级别≥0.8的个体,库尔勒市棉铃虫种群对转Cry1Ac基因棉花仍处于较为敏感阶段。单一大面积种植转Cry1Ac基因棉花可能是库尔勒地区其抗性发展的重要原因。因此,后续还需要继续加强对库尔勒棉铃虫种群的抗性监测。

The purpose of this study was to assay the sensitivity variation of Helicoverpa armigera(Hübner)(Lepidoptera:Noctuidae)to the Cry1 Ac toxin in the Korla cotton growing region,Xinjiang Province.The frequency of alleles conferring resistance to Cry1 Ac toxin and the relative average development rates of H.armigera collected in Korla were assessed from 2013-2019 using the F;and F;offspring,iso-female lines,combined discrimination dose method.From 2013-2019,the mean relative average development rates(RADRs)of H.armigera larvae in the Korla population were 0.328,0.386,0.539,0.572,0.565,0.542 and 0.562,respectively.These results showed that there was an increase in the RADR of H.armigera during 2013-2019 in the Korla populations,suggesting that resistance to Cry1 Ac has increased in H.armigera populations in Korla.The cotton bollworm population in Korla was still sensitive to Cry1 Ac gene in cotton.Bt transgenic cotton planted in a single large area may be an important reason for the development of resistance in Korla region.Therefore,it is necessary to strengthen the resistance monitoring of cotton bollworm.