VPS13C基因作为黑色素瘤患者预后预测指标及免疫细胞浸润相关研究

Studies on VPS13C gene as a prognostic indicator and immune cell infiltraiton in melanoma patients

目的探讨VPS13C基因表达水平对黑色素瘤患者预后的影响及其潜在机制。方法采用GEPIA数据库分析VPS13C基因在肿瘤和正常组织中的表达差异;通过Prognoscan数据库分析VPS13C基因表达与黑色素瘤患者预后的关系;通过TIMER及GEPIA数据库分析VPS13C基因低表达与免疫细胞浸润的相关性。结果相较于正常组织,VPS13C基因在黑色素瘤中低表达,且具有统计学差异(P<0.05);Prognoscan数据库及TCGA数据库分析结果显示VPS13C基因低表达时黑色素瘤患者预后更差。VPS13C高表达的黑色素瘤患者预后更好,生存时间更长;TIMER数据库分析结果显示,在黑色素瘤患者中,多种免疫细胞浸润与VPS13C基因表达水平呈正相关,包括CD8^(+)T细胞,CD4^(+)T细胞等;VPS13C基因表达水平与耗竭前体CD8^(+)T细胞相关基因,Th1细胞相关基因,cDC1相关基因,M1型巨噬细胞相关基因表达水平呈正相关。结论VPS13C基因低表达与黑色素瘤患者不良预后明显相关,可能与耗竭前体CD8^(+)T细胞、Th1细胞、cDC1、M1巨噬细胞高浸润有关。

Melanoma is a highly aggressive cancer in skin and immunotherapy is a new method for melanoma patients.However,only a small population of melanoma patients can respond to immunotherapy.To illustrate this issue,we investigated a new predictive marker for melanoma.We found VPS13C gene expressed highly in normal tissue and is related with melanoma patient survival by using GEPIA database and Prognoscan database.Subsequently,we used TIMER and GEPIA databases to analyze immune cells infiltration,and results showed that immune cells infiltrated more in the melanoma patients with higher expression level of VPS13C gene,including progenitor exhaustion CD8^(+)T cells,cDC1,Th1 and M1 macrophages.Taken together,the high expression of VPS13C in melanoma patients is negatively correlated with the poor prognosis,and the higher infiltration of progenitor exhaustion CD8^(+)T cells,cDC1,Th1 and M1 macrophages play important roles in melanoma patient survival.