血清IL-23、TNF-α表达与溃疡性结肠炎患者中医辨证分型及病变程度的相关性

Correlation of serum IL-23 and TNF-αexpression with TCM syndrome differentiation and lesion degree in patients with ulcerative colitis

目的:探讨血清IL-23、TNF-α表达与溃疡性结肠炎(UC)患者中医辨证分型及病变程度的相关性。方法:选取某院治疗的UC患者90例,根据病变程度将患者分为轻度、中度和重度,其中轻度患者30例,中度患者40例,重度患者20例;按中医辨证分型诊断标准将所有患者分为大肠湿热证、脾虚湿热证、肝郁气滞证、寒热错杂证、脾肾阳虚证,分别为20例、28例、19例、11例、12例。选取同期某院健康者20例作为对照组。采用酶联免疫吸附试验(ELISA)测定研究对象血清IL-23、TNF-α表达水平,多元线性回归分析血清IL-23、TNF-α表达水平对UC病变程度和中医辨证分型的影响。结果:不同病变程度的UC患者和对照组血清IL-23、TNF-α表达水平比较显示:对照组<轻度<中度<重度,差异有统计学意义(F=26.653,88.099,P值均<0.05);不同中医辨证分型的UC患者和对照组血清IL-23、TNF-α表达水平呈现:大肠湿热证>脾虚湿热证>寒热错杂证>肝郁气滞证>脾肾阳虚证>对照组,差异有统计学意义(F=14.365,19.361,P值均<0.05);多元线性回归分析显示,中医辨证分型和病变程度均受血清IL-23、TNF-α表达水平的影响(P<0.005)。结论:不同病变程度、不同中医辨证分型的UC患者间血清IL-23、TNF-α表达水平存在差异性,血清IL-23、TNF-α表达影响UC患者中医辨证分型及病变程度。

Objective:To investigate the correlation of serum interleukin-23(IL-23)and tumor necrosis factor(TNF)in all subjectsα(TNF-α)expression with TCM syndrome differentiation and lesion degree of ulcerative colitis(UC).Methods:90 patients with UC in a hospital were selected and divided into a mild group(30 cases),a moderate group(40 cases)and a severe group(20 cases)according to the degree of lesions.Based on the standard of TCM syndrome differentiation,the patients were diagnosed with large intestine damp heat syndrome(20 cases),spleen deficiency damp heat syndrome(28 cases),liver qi stagnation syndrome(19 cases),cold heat mixed syndrome(11 cases)and spleen kidney yang deficiency syndrome(12 cases).At the same time,a normal control group(20 cases)was set up.Enzyme linked immunosorbent assay(ELISA)was used to detect the levels of serum IL-23 and TNF-α.The expression level was detected by multiple linear regression analysis.Results:The comparison of IL-23 and TNF-αlevels between the control group and the UC patients with different degrees of lesions indicated the result of the control group<mild group<moderate group<severe group,the difference being statistically significant(F=26.653,88.099,all P<0.05).The expression levels of serum IL-23 and TN-αin the control group and UC patients with different TCM syndrome differentiation suggested:large intestine damp heat syndrome>spleen deficiency damp heat syndrome>cold heat mixed syndrome>liver qi stagnation syndrome>spleen kidney yang deficiency syndrome>control group,the difference being statistically significant(F=14.365,19.361,all P<0.05).The results of multiple linear regression analysis showed that TCM syndrome differentiation and the degree of the disease were related to serum IL-23 and TNF-α(P<0.005).Conclusion:There are differences of expression levels in serum IL-23 and TNF-αamong UC patients with different degree of lesion and TCM syndrome differentiations.The expression of serum IL-23 and TNF-αis correlated with TCM syndrome differentiation and the lesion degree of UC.