LncRNA SNHG1对IL-6诱导的皮肤鳞状细胞癌细胞的增殖和迁移的影响

Effect of the LncRNA SNHG1 on the proliferation and metastasis of skin squamous cell carcinoma induced by interleukin-6

目的探讨长链非编码RNA(LncRNA)SNHG1对IL-6诱导的皮肤鳞状细胞癌细胞的增殖和迁移的影响。方法通过qRT-PCR检测SNHG1的相对表达;采用CCK8检测细胞活力;采用Transwell检测细胞的迁移和侵袭能力;采用Western blot检测Ki67、MMP-2、E-cadherin、N-cadherin和Vimentin蛋白表达。结果与正常皮肤细胞系HaCaT,SNHG1在皮肤鳞状细胞癌细胞系A431、SCC13、SCL-1中表达显著增加。不同浓度的IL-6诱导A431细胞后,SNHG1相对表达量、细胞活力、迁移细胞数目和侵袭细胞数目显著增加,及上调Ki67、MMP-2、N-cadherin和Vimentin蛋白表达,下调E-cadherin蛋白表达,呈剂量依赖性。IL-6+si-SNHG1组的SNHG1相对表达、细胞活力、迁移细胞数目、侵袭细胞数目、Ki-67、MMP-2、N-cadherin和Vimentin蛋白表达显著低于IL-6+si-NC组,而E-cadherin蛋白表达显著高于IL-6+si-SNHG1组。结论干扰SNHG1表达可以抑制IL-6诱导的皮肤鳞状细胞癌细胞的增殖、迁移、侵袭和上皮间质转化(EMT)。

Objective To investigate the effects of the long non-coding RNA(LncRNA)SNHG1 on the proliferation and metastasis of skin squamous cell carcinoma induced by interleukin(IL)-6.Methods The relative expression of SNHG1 was detected by qRT-PCR,and cell viability was detected using the cell counting kiT-8 assay.The migration and invasion ability of the cells was detected using the Transwell system,and protein expression of the proliferation marker Ki67,matrix metalloproteinase-2(MMP-2),E-cadherin,N-cadherin and vimentin was detected by Western blot.Results Compared with that in the normal skin cell line HaCaT,the expression of SNHG1 was significantly increased in the skin squamous cell carcinoma lines A431,SCC13 and SCL-1.After A431 cells were treated with different concentrations of IL-6,the relative expression of SNHG1,cell viability,and number of migrating and invading cells increased significantly compared with that in untreated cells.Furthermore,the expression of Ki67,MMP-2,N-cadherin and vimentin protein was upregulated,whereas E-cadherin protein was downregulated after IL-6 treatment and the expression was dose-dependent.The relative expression of SNHG1,cell viability,number of migrating and invasive cells,and expression of Ki-67, MMP-2, N-cadherin, and vimentin proteins in the IL-6+si-SNHG1 group were significantly lower than those in the IL-6+si-NC group and the expression of E-cadherin protein was significantly higher than that in the IL-6+si- SNHG1 group. Conclusions Interference with SNHG1 expression inhibited the proliferation, migration, invasion and epithelial-mesenchymal transition of skin squamous cell carcinoma cells induced by IL-6.