5种采集条件下银杏落叶中莽草酸的含量

Content of shikimic acid in fallen leaves of Ginkgo biloba L.under five conditions

为优选出高含量的莽草酸银杏落叶资源,建立了一种HPLC方法测定银杏落叶中莽草酸的含量.比较树上绿叶与落叶、不同胸径、雌株与雄株、不同采集时间、不同地区等5个条件下莽草酸的含量差异.结果表明莽草酸浓度在0.04-0.15 mg/mL范围内线性关系良好(r>0.999);平均加样回收率为99.21%(N=6,RSD=1.35%).不同采集条件下银杏落叶中莽草酸的含量规律如下:银杏落叶中莽草酸的含量平均值大于树上绿叶;落叶中莽草酸的含量随着胸径的增加而增加,线性相关系数R^(2)为0.673;雌株银杏落叶中莽草酸平均含量大于雄株;与不同采集时间以及不同地区之间未呈现出相关性.本研究建立的莽草酸HPLC测定方法快速、简便,专属性强,准确度高;银杏落叶中莽草酸含量变化有一定规律;结果可为莽草酸资源开发利用提供科学依据.

To optimize the high content of shikimic acid in fallen leaves of Ginkgo biloba L.,a high performance liquid chromatography(HPLC)method was established.The content of shikimic acid in green leaves and fallen leaves,leaves with different diameter at breast height,female and male trees,leaves obtained in different collection times,and leaves obtained from different areas were compared.The results showed that the concentration of shikimic acid had a good linear relationship in the range 0.04-0.15 mg/mL(r>0.999),and the average recovery was 99.21%.The content of shikimic acid in G.biloba leaves under different collection conditions was as follows:the average content of shikimic acid in fallen leaves was higher than that in green leaves on trees.The content of shikimic acid in fallen leaves increased with the increase in DBH levels,and the linear regression R^(2) was 0.673;The average content of shikimic acid in female leaves was higher than that in male trees,and the content of shikimic acid in fallen leaves had no relationship with the collection time or region.Therefore,the HPLC determination method of shikimic acid established in this experiment is rapid,simple,specific,and accurate.The content of shikimic acid in the fallen leaves of G.biloba are rugular,which provides a scientific basis for the development and utilization of shikimic acid resources.