基于重测序的3个自封顶番茄品系基因组变异检测分析

Genomic Variation Detection Analysis of 3 Self-pruning Tomato Lines Based on Resequencing

自封顶类型番茄品种产量与主茎花序数有密切关系,关于控制花序封顶性状基因的研究报道较多,而对控制番茄封顶花序节位的相关研究报道较少。为研究不同自封顶番茄主茎封顶花序节位之间的遗传变异,筛选出参与调控主茎封顶花序节位相关的候选基因。本试验利用重测序技术对3个番茄品系GXF、AXF和815进行变异检测。结果表明,3个样本共检测到5 968 501个SNP和485 114个InDel,与参考基因组比对后共发生33 473个基因变异,将发生在CDS区的变异基因进行GO和KEGG数据库比对,发现主要集中于基础代谢和玉米素的生物合成。通过基因组序列比对分析,筛选获得16个可能是参与调控番茄主茎封顶花序节位的关键基因。研究结果明确了相关基因的变异位点信息,为进一步研究自封顶番茄花序节位的遗传机理奠定了基础。

A close relationship was indicated between the yield of self-pruning tomato varieties and the number of main stem flowers. There were many reports on the genes controlling the traits of inflorescence sealing, but few on the related studies controlling the nodes of inflorescence sealing. To study the genetic variation between different self-pruning tomatoes stem and inflorescence segments, the candidate genes involved in the regulation of inflorescence segments were screened out. In this experiment, the variation of 3 tomatoes strains GXF, AXF, and815 were detected by resequencing technology. The results showed that a total of 5 968 501 SNPs and 485 114 InDel were detected in the three samples, and a total of 33 473 gene mutations were detected after comparison with the reference genome. The GO and KEGG databases were used to compare the mutations in the CDS region,and it was found that they mainly focused on basic metabolism and zein biosynthesis. Through genome sequence alignment analysis, 16 genes that may be involved in the regulation of the terminal segments of the tomato main stem were screened. The results confirmed the mutation locus information of related genes and laid a foundation for further research on the genetic mechanism of inflorescence positions of self-pruning tomatoes.