杧果叶片响应胶孢炭疽病菌侵染的转录组分析

Transcriptome Analysis of Mango Leaves Response to Colletotrichum gloeosporioides Infection

为探讨杧果抵御胶孢炭疽病菌的分子机制,分别以感病和未感病的‘贵妃杧’叶片为材料开展转录组测定和分析。本研究以‘贵妃杧’幼嫩叶片为材料,胶孢炭疽菌接种杧果叶片24 h后,转录组分析(RNA-seq)感病和未感病叶片之间的基因表达差异,共得到序列46 733个,110个差异表达基因中,67个基因表达为上调,43个基因表达为下调。利用GO数据库对差异表达基因进行聚类分析,发现差异表达基因主要聚类在细胞及细胞组分、代谢过程、催化活性等方面。利用KEGG数据库对差异表达基因影响的通路进行注释后发现,胶孢炭疽菌侵染主要影响了牛磺酸和牛磺酸代谢、淀粉、蔗糖代谢等途径中关键基因的表达水平。Autophagy-related protein 8C-like等11个基因的RT-qPCR验证与RNA-seq数据一致,且这些基因在6、12、24 h均能迅速响应胶孢炭疽菌的侵染。另外,三羧酸循环(TCA)过程中琥珀酸脱氢酶的4个亚基也参与了胶孢炭疽菌侵染杧果的过程。本研究从转录组水平上分析了杧果响应胶孢炭疽菌侵染过程中的一些关键基因,也从胶孢炭疽菌的角度分析了琥珀酸脱氢酶4个亚基参与了胶孢炭疽菌的侵染过程,有助于丰富杧果抗炭疽病菌的调控机制以及病原菌TCA循环在侵染过程中作用。

In order to study the molecular mechanism of mango resistance to Colletotrichum gloeosporioides, the transcriptome of infected and uninfected leaves were analyzed. In this study, the difference of gene expression between infected and uninfected leaves of young mango leaves infected with Colletotrichum gloeosporioides for 24 hours was analyzed by transcriptome analysis(RNA-seq), a total of 46 733 sequences and 110 differentially expressed genes were obtained, 67 differentially expressed genes up-regulated and 43 down-regulated genes. By cluster analysis of the differentially expressed genes in GO database, it was found that differentially expressed genes are mainly clustered in the cells and cell components, metabolic processes, catalytic activities, etc. At the same time, we used the KEGG database to annotate the pathway of differentially expressed genes, the infection of Colletotrichum gloeosporioides mainly affected the expression level of key genes in taurine and hypotaurine metabolism and starch, sucrose metabolism. RT-qPCR of 11 genes including Autophagy-related protein 8 C-like was consistent with RNA-seq data, and these genes could rapidly respond to the infection of Colletotrichum gloeosporioides at 6, 12 and 24 h. In addition, four subunits of succinate dehydrogenase gene were involved in the infection of mango during the TCA cycle. In this study, some key genes in response to Colletotrichum gloeosporioides infection in mango were analyzed at transcriptome level. From the point of view of Colletotrichum gloeosporioides, four subunits of succinate dehydrogenase involved in the infection process of Colletotrichum gloeosporioides were also analyzed. It is helpful to enrich the regulatory mechanism of mango anthracnose resistance and the role of pathogen TCA cycle in the infection process.