正常来源CD4^(+)CD25^(+)细胞在小鼠肺癌模型中的抗肿瘤作用

Antitumor Effect of Normal Mice Derived CD4^(+)CD25^(+)Cells in Mice Lung Cancer Model

CD4^(+)CD25^(+)细胞是一群具有免疫抑制活性的T细胞,又称为调节性T细胞(regulatory T cells,Tregs)。肿瘤发生中Tregs会抑制T细胞的活化,促进肿瘤的发生、发展,而正常生理来源的Tregs回输后产生的免疫调节作用是未知的。为探讨正常生理状态下Tregs的免疫调节作用,利用小鼠肺癌模型和细胞移植术,比较正常和荷瘤小鼠CD4^(+)CD25^(+)细胞移植对肿瘤生长、受体小鼠T细胞产生以及肺部病变的影响。结果显示:与对照组相比,移植正常CD4^(+)CD25^(+)细胞的小鼠生存期延长、肿瘤生长缓慢,其外周血与脾脏中T细胞及其亚群含量增加,小鼠肺部无明显病变;而移植荷瘤CD4^(+)CD25^(+)细胞的小鼠生存期缩短、肿瘤生长较快,其外周血与脾脏中T细胞及其亚群含量显著下降,肺部出现更严重弥散灶性病变。这些结果表明,正常来源CD4^(+)CD25^(+)细胞在小鼠肺癌模型中具有抗肿瘤作用。

CD4^(+)CD25^(+)cells are a group of T cells with immunosuppressive activity,which are also known as regulatory T cells(Tregs).Tregs suppress T-cell activation and promote tumorigenesis and progression in tumorigenesis,whereas the immunomodulatory effects produced by Tregs of normal physiological origin upon return transfusion are unknown.To investigate the immunomodulatory effect of Tregs under normal physiological conditions,the effects of tumor growth and T-cell production and lung lesions were compared in normal and tumor-burdened mice of CD4^(+)CD25^(+)cell transplantation by mouse lung cancer model and cell transplantation.The results showed that mice transplanted with normal CD4^(+)CD25^(+)cells had longer survival,slower tumor growth,increased T-cell and their subpopulations in the peripheral blood and spleen,and no significant lesions in the lungs compared with the control group,while mice transplanted with tumor-bearing CD4^(+)CD25^(+)cells had shorter survival,faster tumor growth,significantly decreased T-cell and their subpopulations in the peripheral blood and spleen,and more severe diffuse focal lesions in the lungs.These results indicated that normal-derived CD4^(+)CD25^(+)cells had antitumor effects in the mouse lung cancer model.