‘芙蓉李’糖转运蛋白家族基因鉴定及表达分析

Identification and Expression Analysis of Sugar Transporter Family Genes in ‘Furongli’(Prunus salicina)

基于‘芙蓉李’(Prunus salicina)基因组数据,采用生物信息学分析方法鉴定糖转运蛋白家族基因,并对其蛋白质理化特性、亚细胞定位、系统进化树、蛋白质保守结构域以及表达模式进行分析。共鉴定出49个该家族基因,根据保守结构域和系统发育分析将其分为7个亚家族。蛋白质介于313~948个氨基酸之间,预测定位于质膜或液泡膜;所有的蛋白都含有保守的糖转运体结构域(PF00083),其中大多数含有12个跨膜螺旋。通过比对‘芙蓉李’果实发育过程的RNA-seq数据,对49个基因进行表达谱分析,其中15个在果实不同发育阶段差异表达;qRT-PCR分析表明,15个差异表达基因中有9个基因与果实葡萄糖和蔗糖含量显著正/负相关,其中1个肌醇转运蛋白亚家族基因(PsINT4)、1个糖转运蛋白亚家族基因(PsSTP11)、1个糖促进蛋白亚家族基因(PsSFP5)和1个液泡单糖转运蛋白亚家族基因(PsTMT1)在‘芙蓉李’果实发育过程中具有较高的表达量,可能在‘芙蓉李’果实发育和成熟过程中对糖分积累起重要作用。

A comprehensive analysis was carried out to identify sugar transporter(ST)genes and dissect their phylogenetic relationships,and to investigate their expression patterns during fruit development in‘Furongli’(Prunus salicina Lindl.)based on its genome data. A total of 49 ST genes were identified in the‘Furongli’genome and they were divided into seven subfamilies according to conserved domains and phylogenetic analysis. Bioinformatics analysis results showed that the length of PsSTs ranged from 313 to 948 aa,and all of them were predicted to locate in membrane. All the PsSTs contained the conserved sugar transporter domain(PF00083),and the majority of them contained 12 transmembrane helices(TMHs). Expression profiles of the 49 PsST genes during fruit development derived from RNA-Seq data indicated that 15 PsST genes were differentially expressed at different developmental stages. qRT-PCR analysis showed that nine of the 15 differentially expressed genes were significantly correlated with glucose and sucrose content in‘Furongli’fruit. Among them,one inositol transporter gene(PsINT4),one sugar transporter protein gene(PsSTP11),one sugar facilitator protein gene(PsSFP5)and one tonoplast monosaccharide transporter gene(PsTMT1)were identified as candidate genes,which may play important roles in sugar accumulation during the development and ripening of‘Furongli’fruit.