大白菜LEA家族基因的鉴定及其部分成员在低温胁迫下的表达分析

Identification of LEA Family and Expression Analysis of Some Members Under Low-temperature Stress in Chinese Cabbage

基于大白菜全基因组数据对LEA家族基因进行全基因组鉴定与生物信息学分析,研究其序列特征、基因结构、启动子顺式作用元件、染色体定位、进化关系以及在不同组织中和低温胁迫下的表达模式。从大白菜基因组中总共鉴定出65个LEA家族基因成员,根据系统发育与保守基序分析,其可分为8个组;内含子较少,且不均匀地分布在10条染色体上,另有3个成员位于Scaffold上。启动子顺式作用元件分析显示,有29个(44.6%)成员拥有低温响应元件,除此之外,光响应元件和激素响应元件占比较高。共线性分析显示,大白菜BrLEA与拟南芥AtLEA同源性较高,并在进化中较为保守。大白菜不同组织转录组数据表明,BrLEA基因在大白菜不同组织中的表达存在组织特异性。对筛选出的13个家族成员进行低温胁迫表达分析表明,受低温胁迫表达量都有所升高。

This study was based on the whole genome data of Chinese cabbage to identify and bioinformatics the late embryogenesis abundant protein(LEA)family genes. The sequence characteristics,gene structure,promoter cis-acting elements,chromosome localization,evolutionary relationships,and expression patterns in different tissues and under low temperature stress were investigated. The results showed that 65 family members were identified from the Chinese cabbage genome and could be divided into eight groups according to phylogenetic and conserved motif analysis. Fewer introns are prevalent and unevenly distributed on ten chromosomes,with three other members located on the Scaffold. The promoter cis-acting element analysis showed that 29 members(44.6%)had low temperature response elements.In addition,the proportion of light response elements and hormone response elements was the highest. The collinearity analysis showed that BrLEAs and AtLEAs had higher homology and were conservative in evolution. Analysis of transcriptome data of different tissues of Chinese cabbage found that the expression of BrLEA genes in different tissues was different and had tissue specificity. The expression of 13 family members under low temperature stress family members showed that the expression of low temperature stress increased.