纳米金修饰锌基金属有机框架用于过氧化氢的检测研究

Construction of enzyme-free electrochemical sensor for hydrogen peroxide detection in serum based on Au NPs/metal organic framework ZIF-8

本研究利用溶剂热法制备锌基金属有机框架材料(ZIF-8),再通过电沉积法将金纳米颗粒(Au NPs)负载到ZIF-8上,构建无酶电化学传感器(Au NPs/ZIF-8/GCE)用于过氧化氢(H_(2)O_(2))的电化学测定。通过扫描电子显微镜(SEM)和透射电子显微镜(TEM)对修饰材料的形貌进行了表征。实验采用循环伏安法(CV)研究该传感器的电化学行为,并进一步研究不同扫速和不同H_(2)O_(2)浓度对修饰电极催化性能的影响。结果表明,该传感器对H_(2)O_(2)的还原具有很好的电催化活性。在最佳条件下,采用计时电流法(i-t)测定H_(2)O_(2)浓度在0.01~59 mmol·L^(-1)范围内呈良好的线性关系,检出限为2.20μmol·L^(-1)。该传感器制作简单,选择性、重复性和稳定性好,将其用于血清样品中H_(2)O_(2)含量检测,加标回收率在98.6%~102.7%之间,为生物样品中H_(2)O_(2)的简单快捷检测提供了需要。

In this work,ZIF-8,a kind of metal organic frameworks(MOFs),was synthesized using the solvothermal method,and the gold nanoparticles(Au NPs)were loaded on ZIF-8 through electrodeposition to prepare the hydrogen peroxide enzyme-free electrochemical sensors(Au NPs/ZIF-8/GCE).The morphology of the modified materials was characterized by scanning electron microscopy(SEM)and transmission electron microscopy(TEM).The electrochemical behavior of the biosensor was evaluated by cyclic voltammetry(CV),and the effects of scanning rate and different H_(2)O_(2)concentrations on electrocatalytic performance were further investigated.Results showed that the biosensor has a good electrocatalytic activity for H_(2)O_(2)reduction.Under the optimal conditions,the concentration of H_(2)O_(2)in the linear range was from 0.01 to 59 mmol·L^(-1)with the limit of detection 2.20μmol·L^(-1).The biosensor has a simple production procedure,excellent selectivity,good reproducibility and good stability,which was used to detect H_(2)O_(2)in serum samples and provides the need for simple and quick detection of H_(2)O_(2)in biological samples.