新化合物Elixir-X对肝切及辐射所致衰老小鼠的抗衰老作用

Anti-aging effect of Elixir-X in mice induced by hepatectomy and radiation

目的探讨蜂巢提取物Elixir-X对自然衰老及辐射衰老小鼠的影响,为Elixir-X的进一步研发提供药理学基础。方法采用小鼠肝脏切除术建立自然衰老小鼠模型,将其分为空白对照组、Elixir-X组、白藜芦醇组及模型组,连续给药7 d后,检测小鼠70%肝脏切除后的再生过程中,衰老细胞、肝脏功能恢复与肝脏再生的变化,观察Elixir-X对自然衰老的影响;采用^(60)CO辐射法建立辐射衰老小鼠模型,将其分为模型组、空白对照组、白藜芦醇组、Elixir-X高、低剂量组,通过检测小鼠肠隐窝干细胞再生、小肠绒毛结构以及衰老细胞的变化,对比不同剂量Elixir-X对辐射后衰老的影响。结果在肝脏切除术的小鼠模型中,Elixir-X组的肝功能水平明显低于模型组的肝脏自然再生小鼠,显著促进了肝功能恢复(P<0.05);同时与模型组相比,Elixir-X组清除了肝脏切除后再生过程中产生的衰老细胞(P<0.05),Ki-67的着色斑块明显增加,促进肝脏的再生,且肝血窦的扫描电镜影像结果中Elixir-X组肝血窦再生加快。^(60)CO辐照小鼠模型中,与模型组相比,Elixir-X组有效促进辐照后肝脏功能恢复(P<0.05),Elixir-X高剂量组体重也恢复到正常水平,与空白对照组无显著差异;与模型组相比,Elixir-X低剂量组小肠的β-Gal着色细胞减少,Elixir-X高剂量组甚至能更有效地减少衰老细胞(P<0.05);小肠HE染色的结果中,Elixir-X组小肠绒毛结构得到恢复;Ki-67的IHC染色中,与模型组相比,Elixir-X组着色板块更多,且着色区域都在肠隐窝干细胞位置。结论Elixir-X能有效清除肝脏切除术引起的自然衰老与辐射引起的损伤性衰老细胞,对衰老具有显著地抑制作用。

Objective To explore the effect of honeycomb extract Elixir-X on natural aging and radiation aging mice,and provide a pharmacological basis for the further development of Elixir-X.Methods Mice were divided into blank control group,Elixir x group,resveratrol group and model group.The changes of senescent cells,liver function recovery and liver regeneration during regeneration after 70%liver resection in mice were detected,to observe the effect of Elixir-X on natural senescence.The mice model of radiation aging was established using ^(c)o-60 coefficient CO radiation method and divided into model group,blank control group,resveratrol group,high dose and low dose Elixir-X group.The changes of intestinal crypt stem cell regeneration,villus structure and senescent cells were detected.The effects of different doses of Elixir-X on senescence after radiation were compared.Results In hepatectomy mice,the liver function level of Elxir-X treatment group was significantly lower than that of normal liver regeneration mice,and significantly promoted liver function recovery(P<0.05).Compared with the model group,the senescent cells in the Elixir-X group were removed(P<0.05),and the staining plaque of Ki-67 was significantly increased,which promoted the regeneration of liver.Compared with model group,Pressume 60 Species in mice irradiated with Elixir-X can effectively promote liver function recovery(P<0.05),and body weight of Elixir-X group recovered to normal level,no significant difference was found between the two groups(P>0.05).Compared with model group,β-Gal staining cells were decreased in Elixir-X-L group,and even decreased senescent cells more effectively in Elixir-X-H group(P<0.05).The small intestinal villi were recovered in the treatment group of Elixir-X by HE staining.In IHC staining of Ki-67,there were more staining plates in the Elixir-X group compared with the model group,and the staining areas were all in the position of intestinal crypt stem cells.Conclusion Elixir X can effectively eliminate the natural aging caused by liver resection and the damaged senescent cells caused by radiation,and it has a significant inhibition effect on aging.