炉甘石氧化锌洗剂中苯酚含量测定研究

Research on the Determination of Phenol in Calamine Zinc Oxide Lotion

目的:建立炉甘石氧化锌洗剂中苯酚的含量测定方法。方法:采用高效液相色谱法(HPLC),色谱柱为Welch Xtimate■C18(5μm,4.6 mm×150 mm),以甲醇-水(40:60)为流动相进行等度洗脱,流速1.0 mL·min^(-1),柱温30℃,进样量10μL,检测波长270 nm。结果:苯酚在62.08~434.56μg·mL^(-1)线性关系良好(r>0.999),加样回收率为100.46%,相对标准偏差(RSD)为0.28%。结论:本研究建立的方法简便、快速、准确,重现性好、精密度高。可用于炉甘石氧化锌洗剂中苯酚的质量控制。

Objective To establish a method for the determination of phenol in calamine zinc oxide lotion.Methods High performance liquid chromatography(HPLC)was used,the chromatographic column was Welch Xtimate■C18(5μm,4.6 mm×150 mm),methanol-water(40:60)was used as the mobile phase for isocratic elution,the flow rate was 1.0 mL·min;,column temperature was 30℃ and injection volume was 10μL.The detection wavelength was 270 nm.Results Phenol had a good linear relationship in the range of 62.08~434.56μg·mL^(-1)(r>0.999),the sample recovery rate was 100.46%,and the relative standard deviation(RSD)was 0.28%.Conclusion The method established in this study is simple,fast,accurate,reproducible,and highly precise.It can be used for the quality control of phenol in calamine zinc oxide lotion.