摘要
由于天然水体中合成的四氧化三铁纳米颗粒(Fe_(3)O_(4)NPs)含量未有可靠数据,因此就其安全阈值开展研究。本研究以模式动物斑马鱼为受试生物,将2 hpf的斑马鱼胚胎暴露在含有不同浓度Fe_(3)O_(4)NPs的沉积物中,96 hpf后考察其对斑马鱼胚胎发育和氧化应激水平的影响。结果显示,随着沉积物中Fe_(3)O_(4)NPs浓度的增加,斑马鱼胚胎及幼鱼的死亡率、畸形率呈现上升趋势,孵化率则呈现下降趋势。斑马鱼胚胎的死亡率、孵化率在0.8 mg·g^(-1)Fe_(3)O_(4)NPs的暴露下开始显著下降(P<0.05),畸形率则在0.4 mg·g^(-1)Fe_(3)O_(4)NPs暴露下开始显著上升(P<0.05)。96 hpf斑马鱼幼鱼体内活性氧(ROS)和丙二醛(MDA)含量随着沉积物中Fe_(3)O_(4)NPs浓度的增加而升高。超氧化物歧化酶(SOD)活性在1.6 mg·g^(-1)和3.2 mg·g^(-1)Fe_(3)O_(4)NPs暴露下显著下降(P<0.05),总抗氧化能力(T-AOC)在Fe_(3)O_(4)NPs最高浓度组(3.2 mg·g^(-1))显著下降(P<0.05)。随着沉积物中Fe_(3)O_(4)NPs浓度的增加,96 hpf的斑马鱼幼鱼体内Fe_(3)O_(4)NPs含量呈上升趋势,从0.4 mg·g^(-1)Fe_(3)O_(4)NPs开始与对照组相比具有显著差异(P<0.05)。因此,沉积物中Fe_(3)O_(4)NPs含量≤0.2 mg·g^(-1)是安全的,≥0.4 mg·g^(-1)是不安全的,沉积物中Fe_(3)O_(4)NPs的安全阈值范围是0.2~0.4 mg·g^(-1)。
Since there is no available analytical data for the content of synthesized Fe_(3)O_(4)nanoparticles(NPs)in natural water,the safety threshold was studied in this paper.A model animal zebrafish was used as the test organism,and 2 hpf zebrafish embryos were exposed to sediments containing different concentrations of Fe_(3)O_(4)NPs.After exposure until 96 hpf,the mortality and malformation rates of zebrafish embryos increased and the hatching rate decreased with the increase of Fe 3 O 4 NPs in the artificial sediment.Fe_(3)O_(4)NPs decreased the hatching rate of zebrafish embryos significantly(P<0.05)at the exposure concentration of 0.8 mg·g^(-1),and increased the malformation rates significantly(P<0.05)at 0.4 mg·g^(-1).With the increase of Fe_(3)O_(4)NPs content in sediment,the reactive oxygen species(ROS)and malondialdehyde(MDA)levels in zebrafish juvenile were increased,while the superoxide dismutase(SOD)activity was decreased at 1.6 mg·g^(-1)and 3.2 mg·g^(-1)(P<0.05).The total antioxidant capacity(T-AOC)reduced significantly in the highest concentration group(3.2 mg·g^(-1))(P<0.05).Iron accumulation in zebrafish showed an upward trend,and it was significantly higher at 0.4 mg·g^(-1)than that in the control group(P<0.05).The results indicate that it is safe for the content of Fe_(3)O_(4)NPs in sediments to be less than or equal to 0.2 mg·g^(-1),and higher than or equal to 0.4 mg·g^(-1)is unsafe.Therefore,the concentration range for the safety threshold of Fe_(3)O_(4)NPs in the sediment is from 0.2 mg·g^(-1)to 0.4 mg·g^(-1).
作者
文婷
隋彦伯
周雅娜
张赟
魏晨曦
Wen Ting;Sui Yanbo;Zhou Yana;Zhang Yun;Wei Chenxi(State Key Laboratory of Freshwater Fish Developmental Biology,College of Life Sciences,Hunan Normal University,Changsha 410081,China)
出处
《生态毒理学报》
CAS
CSCD
北大核心
2021年第6期191-200,共10页
Asian Journal of Ecotoxicology
基金
淡水鱼发育生物学国家重点实验室自主课题(20180118)
湖南师范大学青年科学基金资助项目(31401)。