牛miR-144靶基因预测与组织表达分析

Prediction of target genes and tissue expression analysis of miR-144 in cattle

为研究miR-144的靶基因与miR-144在牛不同组织中的表达规律,预测其对肌肉生长发育的调节机制,对miR-144在各物种间的保守性、潜在靶基因及其富集通路进行分析,通过qRT-PCR方法检测牛不同组织中miR-144的表达。结果表明,miR-144成熟序列在各物种间保守性较高;富集分析发现,靶基因显著富集于血管平滑肌收缩、cGMP-PKG、cAMP等与肌肉发育相关通路中。qRT-PCR结果显示,miR-144在肝中的表达量最高,在皮下脂肪中的表达量最低;miR-144在腿肌和心中的表达差异不显著(P>0.05),但在肝中的表达显著(P<0.05)高于腿肌和心;miR-144在背最长肌中的表达显著(P<0.05)高于瘤胃和皮下脂肪,而瘤胃和皮下脂肪组织间表达差异不显著(P>0.05)。

To study the target genes of miR-144 and their expression patterns in different tissues of cattle,and to predict the regulatory mechanism of miR-144 on muscle growth and development,the conservation,potential target genes and their enrichment pathways of miR-144 among different species were analyzed,and expression of miR-144 in different tissues of cattle was detected by qRT-PCR.The results showed that mature sequence of miR-144 was highly conserved among species;Enrichment analysis showed that target genes were significantly enriched in vascular smooth muscle contraction,cGMP-PKG,cAMP and other pathways related to muscle development.qRT-PCR results showed that miR-144 had the highest expression in liver and the lowest expression in subcutaneous fat;There was no significant difference(P>0.05)in the expression of miR-144 in leg muscle and heart,but the expression of miR-144 in liver was significantly(P<0.05)higher than that in leg muscle and heart;The expression of miR-144 in longissimus dorsi was significantly(P<0.05)higher than that in rumen and subcutaneous adipose,but there was no significant difference(P>0.05)between rumen and subcutaneous adipose.