IgA肾病患者IgA1分子糖基化异常与Th17细胞的相关性分析

Correlation of Abnormal IgA1 Molecular Glycosylation with Th17 Cells in Patients with IgA Nephropathy

目的:探讨IgA肾病患者IgA1分子糖基化异常与Th17细胞的相关性分析。方法:选取50例IgA肾病患者(IgA肾病组)与50例健康体检人员(健康对照组)的临床资料进行回顾性分析,选取时间为2018年2月至2020年5月。比较Th17细胞、Treg细胞、Gd-IgA1、TGF-β、IL-6、IL-17、IL-21、IL-23、血肌酐、白蛋白及尿蛋白定量;采用Pearson法分析Gd-IgA1与Th17细胞、Treg细胞的相关性,以及细胞因子与Gd-IgA1的相关性。结果:IgA肾病组的Th17细胞、Th17/Treg、Gd-IgA1、IL-6、IL-17、IL-21、IL-23、血肌酐、尿蛋白定量均高于健康对照组,而Treg细胞、TGF-β、白蛋白低于健康对照组(P<0.05)。Pearson相关性显示,Gd-IgA1与Th17细胞、Th17/Treg呈正相关(r=0.737/0.810,P<0.05),与Treg细胞呈负相关(r=-0.487,P<0.05)。Gd-IgA1与TGF-β呈负相关(r=-0.260,P<0.05),与IL-6、IL-17、IL-21、IL-23呈正相关(r=0.796/0.776/0.714/0.819,P<0.05)。结论:在IgA肾病患者中,Th17细胞与Gd-IgA1呈正相关,可通过评估Th17细胞表达量,为临床诊治提供依据。

Objective:To investigate the correlation between abnormal glycosylation of IgA1 molecule and Th17 cells in patients with IgA nephropathy.Methods:The clinical data of 50 patients with IgA nephropathy(IgA nephropathy group)and 50 healthy people(healthy control group)were selected for retrospective analysis.The patients’admission period was from February 2018 to May 2020.Th17 cells,Treg cells,Gd-IgA1,TGF-β,IL-6,IL-17,IL-21,IL-23,serum creatinine,albumin and urine protein quantification were compared;Pearson method was used to analyze Gd-IgA1 and Th17 correlation of cells,Treg cells,and correlation of cytokines with Gd-IgA1.Results:Th17 cells,Th17/Treg,Gd-IgA1,IL-6,IL-17,IL-21,IL-23,serum creatinine and urine protein in the IgA nephropathy group were all higher than those in the healthy control group,while Treg cells,TGF-βand albumin were lower than the healthy control group(P<0.05).Pearson correlation showed that Gd-IgA1 was positively correlated with Th17 cells and Th17/Treg(r=0.737/0.810,P<0.05),and negatively correlated with Treg cells(r=-0.487,P<0.05).Gd-IgA1 was negatively correlated with TGF-β(r=-0.260,P<0.05),and positively correlated with IL-6,IL-17,IL-21,and IL-23(r=0.796/0.776/0.714/0.819,P<0.05).Conclusion:In patients with IgA nephropathy,Th17 cells are positively correlated with Gd-IgA1,and the expression of Th17 cells can be evaluated to provide a basis for clinical diagnosis and treatment.