芝麻素对大鼠骨质疏松症的作用研究

Study on the effect of sesamin on osteoporosis in rats

目的在大鼠模型中探讨芝麻素对骨质结构改变的作用及对骨钙素和I型胶原表达的影响。方法建立去卵巢(ovariectomized,OVX)大鼠模型,给予芝麻素(sesamin,80 mg/kg)灌胃作为干预措施并同时建立对照组。应用显微计算机断层扫描(Micro-CT)对大鼠股骨进行分析,并应用免疫组化及免疫荧光检测大鼠股骨中的骨钙素(osteocalcin,OCN)水平以及I型胶原表达。结果本实验研究条件下,与OVX组相比,OVX+S骨密度(bone mineral density,BMD)增加、小梁间距(trabecular spacing,Tb.Sp)降低,同时,干预组骨钙素和I型胶原表达显著增强(P<0.05)。结论芝麻素可有效促进去卵巢大鼠骨结构重塑,对大鼠骨质疏松症有积极的治疗和预防作用。

Objective To investigate the effect of sesamin on the changes of bone structure and the expression of osteocalcin and type I collagen in the rat model.Methods Ovariectomized(OVX)rat model was established.Rats received sesamin(80 mg/kg)by intragastric administration as an intervention and a control group was established at the same time.The femur of rats was analyzed using micro-computed tomography(micro-CT).The content of osteocalcin(OCN)and the expression of type I collagen in the femur were detected with immunohistochemistry and immunofluorescence.Results Compared to those in the OVX group,bone mineral density(BMD)of OVX+S increased and the trabecular spacing(Tb.Sp)decreased in the experimental group.At the same time,the expressions of osteocalcin and type I collagen in the intervention group was significantly higher than those in the intervention group(P<0.05).Conclusion Sesamin effectively promotes the remodeling of bone structure in ovariectomized rats and has a positive effect on the treatment and prevention of osteoporosis in rats.