40%环丙唑醇悬浮剂高效液相色谱分析

Analysis of cyproconazole 40% SC by HPLC

[目的]建立一种对40%环丙唑醇悬浮剂进行分离和测定的高效液相色谱分析方法。[方法]采用高效液相色谱法,以甲醇-超纯水为流动相,使用SunFire C_(18)为填料的不锈钢色谱柱和PDA检测器,在220 nm波长下对供试物中的环丙唑醇进行分离和定量分析。[结果]该分析方法的环丙唑醇线性相关系数R^(2)为0.999,标准偏差为0.10,变异系数为0.25%。平均回收率为99.39%。[结论]该检测方法方便、快速,分离效果好,线性关系、准确度和精密度均符合要求,可用于原药或制剂中环丙唑醇的定量分析。

[Aims]This study aims to establish an analytical method for separation and quantitative analysis of cyproconazole 40% SC by HPLC. [Methods]The separation and quantitative analysis of cyproconazole samples were conducted by HPLC and PDA detector at 220 nm with the mixture of methanol and ultra-pure water as mobile phase,and with SunFire C;column as fixed phase. [Results]The results showed that the linear correlation coefficients of cyproconazole was 0.999, the standard deviation was 0.10, the coefficients of variation was 0.25%, and the average recoveries was 99.39%. [Conclusions]This method has the advantages of simple, rapid, and good separation effect, the linear relations, accuracy, and precision, which can meet the requirement, it is suitable for quantitative analysis of cyproconazole in technical material and preparation.