摘要
前期工作通过组学数据分析、蛋白分离纯化以及质谱鉴定,发现一些基因可能参与调控柠檬烯微生物转化生成α-松油醇的过程,比如PDIDSM_85260和PDIDSM_08010等。为进一步验证这些基因的功能,利用RNAi技术构建了基因PDIDSM_85260和PDIDSM_08010的干涉载体,并通过农杆菌介导转化到指状青霉DSM62840的菌丝中,经过一系列实验的验证,得到干涉菌株,并对这两个基因的功能进行研究。结果表明,与野生型菌株相比,基因PDIDSM_85260和PDIDSM_08010的干涉菌株通过微生物转化得到的α-松油醇含量均有不同程度的下降,说明基因PDIDSM_85260和PDIDSM_08010可能参与调控柠檬烯的生物转化过程。
Our previous results have shown that some genes such as PDIDSM_85260 and PDIDSM_08010 may be partly responsible for limonene biotransformation toα-terpineol as determined by analysis of genomic and transcriptomic data,protein separation and purification,and mass spectrometry.In order to explore the biological functions of the genes,RNA interference(RNAi)vectors for PDIDSM_85260 and PDIDSM_08010 were constructed and transformed into Penicillium digitatum DSM62840 by Agrobacterium tumefaciens-mediated transformation.RANi strains were obtained by a series of experiments,and the functions of the two genes were analyzed in P.digitatum DSM62840.It was found that the amount ofα-terpineol produced by the silenced transformants obviously decreased compared to the wild-type strain,suggesting that PDIDSM_85260 and PDIDSM_08010 may participate in limonene biotransformation toα-terpineol.
作者
张璐璐
范刚
李晓
任婧楠
潘思轶
黄文
何进
ZHANG Lulu;FAN Gang;LI Xiao;REN Jingnan;PAN Siyi;HUANG Wen;HE Jin(College of Food Science and Technology,Henan University of Technology,Zhengzhou 450001,China;Key Laboratory of Environment Correlative Dietology,Ministry of Education,College of Food Science and Technology,Huazhong Agricultural University,Wuhan 430070,China;College of Life Science and Technology,Huazhong Agricultural University,Wuhan 430070,China)
出处
《食品科学》
EI
CAS
CSCD
北大核心
2022年第6期141-148,共8页
Food Science
基金
国家自然科学基金面上项目(31671824)
国家自然科学基金青年科学基金项目(32101868)。
