摘要
通过环保、低成本的混合法制备氧化锆(ZrO2)和羟基磷灰石(HA)增强钛基支架材料,该材料具有较低的弹性模量(E)、足够的物理、电化学和生物学性能。研究羟基磷灰石和氧化锆体积分数的变化对支架力学性能、腐蚀性能和抗菌性能的影响。采用扫描电子显微镜结合电子色散光谱和X射线衍射对支架进行表征,通过压缩试验检测材料的力学性能及其详细的断裂机制,用电化学法测试样品在37℃模拟体液中的体外腐蚀敏感性,通过比较大肠杆菌和金黄色葡萄球菌的抑菌面积进行抑菌试验。结果表明,随着HA:ZrO_(2)体积分数比例的增加,支架的力学强度降低,当其比例为6:4时,复合支架的E最低(6.61GPa)。纯钛、HA:ZrO_(2)体积分数比例为3:2和6:4的支架材料,其腐蚀电流密度(J_(corr))分别为21、337和504μA/cm^(2)。体积分数比例为6:4的支架对金黄色葡萄球菌的抑菌能力优于其他支架。
Ti-based scaffolds reinforced with zirconia and hydroxyapatite were produced successfully by a hybrid method with an eco-friendliness and low cost to obtain low elastic modulus(E) with sufficient physical, electrochemical and biological properties. The effect of simultaneous modification of the volume fraction of hydroxyapatite(HA) and zirconia(ZrO_(2)) on scaffolds was investigated in terms of mechanical, corrosive, and antibacterial properties. Scanning electron microscopy with attached electron dispersive spectroscopy and X-ray diffraction were used for the characterization of scaffolds. Compression and electrochemical tests were performed to determine mechanical properties with detailed fracture mechanism and in-vitro corrosion susceptibility to simulated body fluid at 37 ℃,respectively. Antibacterial tests were carried out by comparing the inhibition areas of E.coli and S.aureus bacteria. It was observed that the mechanical strength of the scaffolds decreased with increasing HA:ZrO_(2)volume fraction ratio.The lowest E was achieved(6.61 GPa) in 6:4 HA:ZrO_(2)composite scaffolds. Corrosion current density(J_(corr)) values were calculated to be 21, 337, and 504 μ A/cm^(2) for unreinforced Ti, 3:2 and 6:4 HA:ZrO_(2)reinforced scaffolds,respectively. The inhibition capacity of the 6:4 reinforced composite scaffold was found to be more effective against S.aureus bacteria than other scaffolds.
基金
the financial supports from the Research Fund of Atatürk University, Turkey (No. FDK-2019-7281)。
关键词
钛生物复合材料
羟基磷灰石
氧化锆
支架
力学性能
体外腐蚀
titanium biocomposite
hydroxyapatite
zirconia
scaffold
mechanical properties
in-vitro corrosion
