摘要
目的通过超高效液相-四极杆飞行时间质谱(UHPLC-Q-TOF/MS)联用技术定性分析中成药复方金钱草颗粒中的主要化学成分。方法色谱分离采用XBridge BEH C18柱(2.1 mm×100 mm,2.5μm),流动相由0.1%甲酸水和0.1%甲酸乙腈组成,梯度洗脱,流速为0.4 ml/min;质谱定性采用四极杆飞行时间质谱,正离子模式扫描。结果在优化的液质联用条件下,鉴定出复方金钱草颗粒中47个成分,并通过软件计算区分了其中的同分异构体,且对其药材来源进行了归属。结论本研究通过UHPLC-Q-TOF/MS联用技术,为鉴定中成药复方金钱草颗粒中的化学成分建立了一种快速、高效的分析方法。
Objective To qualitatively analyze the main chemical components in compound Jinqiancao granules by ultra high performance liquid chromatography-quadrupole time-of-flight mass spectrometry(UHPLC-Q-TOF/MS).Methods XBridge BEH C18 column(2.1 mm×100 mm,2.5μm)was used for chromatographic separation.The mobile phase was composed of 0.1%formic acid water and 0.1%formic acid-acetonitrile,gradient elution,and the flow rate was 0.4 ml/min.Mass spectrometry was characterized by Quadrupole time-of-flight mass spectrometry(Q-TOF/MS)and positive ion mode scanning.Results Under the optimized LC/MS condition,47 components in compound Jinqiancao granules were identified.The isomers were distinguished by software calculation.The source of medicinal materials was assigned.Conclusion A rapid and efficient analytical method was established for the identification of chemical components in compound Jinqiancao granules by UHPLC-Q-TOF/MS.
作者
张哲
孙卓然
潘鹏超
陈啸飞
柴逸峰
ZHANG Zhe;SUN Zhuoran;PAN Pengchao;CHEN Xiaofei;CHAI Yifeng(Department of Pharmacy,No.971 Naval Hospital of the PLA,Qingdao 266071,China;School of Pharmacy,Naval Medical University,Shanghai 200433,China;The First Naval Hospital of Southern Theater Command of the PLA,Zhanjiang 524005,China)
出处
《药学实践杂志》
CAS
2022年第2期146-151,156,共7页
Journal of Pharmaceutical Practice
