发酵巴戟天中蒽醌提取及其抗氧化与降血糖活性

Extraction of Anthraquinone from Fermented Morinda officinalis and Its Antioxidant and Hypoglycemic Activities

目的:优化发酵巴戟天蒽醌的提取工艺,并评价其抗氧化和降血糖活性。方法:以蒽醌提取率为指标,通过响应面法确定最佳提取工艺;测定蒽醌对DPPH自由基、OH自由基和超氧阴离子的清除能力来评价体外抗氧化活性;测定蒽醌对线虫寿命和运动能力的影响来评价体内抗氧化活性;测定蒽醌对α-葡萄糖苷酶和α-淀粉酶的抑制率来评价体外降血糖活性;测定蒽醌对IR-HepG2细胞葡萄糖消耗量的影响来评价体内降血糖活性。结果:蒽醌最佳提取工艺为乙醇体积分数45%、料液比1:41(g/mL)、提取温度65℃、提取时间1.60 h,在此工艺下蒽醌提取率为90.37%。蒽醌在体外对DPPH自由基、羟自由基和超氧阴离子具有良好的清除能力;在体内能显著延长线虫的寿命(P<0.05),增强线虫的运动能力。蒽醌对α-淀粉酶和α-葡萄糖苷酶的IC50值分别为0.588 mg/mL和0.575 mg/mL;在1.2 mg/mL蒽醌组中IR-HepG2细胞葡萄糖消耗量较模型组细胞极显著提高47.42%(P<0.01)。结论:发酵巴戟天蒽醌具有良好的抗氧化和降血糖活性,为治疗糖尿病的天然药物的开发提供基础数据。

Objective:Optimization of the extraction process of anthraquinone from fermented Morinda officinalis and investigated its antioxidant and hypoglycemic activities.Methods:Taking the extraction ratio of anthraquinone as the evaluation index,the optimal conditions were obtained by response surface methodology.In vitro,the antioxidant activity was evaluated by determining the scavenging ability of anthraquinone against DPPH free radical,hydroxyl free radical and superoxide anion assays.In vivo,the antioxidant activity was evaluated by measuring the effect of anthraquinone on the life span and motility of Caenorhabditis elegans.The hypoglycemic activity in vitro was evaluated by determining the inhibition rate of anthraquinone against alpha-glucosidase and alpha-amylase.The hypoglycemic activity in vivo was evaluated by measuring the effect of anthraquinone on glucose consumption of IR-HepG2 cells.Results:The optimal conditions were obtained as follows:ethanol concentration,45%;solid-to-solvent ratio,1:41(g/mL);temperature,65℃;and time,1.60 h.Under these conditions,the extraction ratio of anthraquinone was 90.37%.In the antioxidant assay in vitro,anthraquinone exhibited good scavenging ability against DPPH free radicals,hydroxyl free radicals,and superoxide anions.In the antioxidant assay in vivo,anthraquinone significantly prolonged the life span(P<0.05),and enhanced the motility of Caenorhabditis elegans(P<0.05).The IC50 values of anthraquinone on alpha-amylase and alpha-glucosidase were 0.588 and 0.575 mg/mL,respectively.Glucose consumption of IR-HepG2 cells in the anthraquinone group(1.2 mg/mL)was increased by 47.42%compared with the model group.Conclusion:The anthraquinone from fermented Morinda officinalis exhibited good antioxidant and hypoglycemic activities,facilitating the development of natural therapeutic agents for diabetes treatment.