白族药用植物鞍叶羊蹄甲对小鼠的急性毒性研究

Study on Acute Toxicity of the Bai Medicinal Plant Bauhinia brachycarpa on Mice

目的:研究鞍叶羊蹄甲提取物对小鼠的急性毒性作用。方法:采用最大给药量法进行急性毒性实验。给小鼠灌胃鞍叶羊蹄甲提取物后,连续观察14 d。期间称量小鼠体质量;采用自主活动实验、平衡木实验和疲劳转棒实验评估小鼠的行为学。给药后第14天处死小鼠,测定脏器指数和血液生化指标,并观察组织病理学变化。结果:与溶剂对照组比较,鞍叶羊蹄甲组小鼠体质量无明显变化;给药后第1天,小鼠自主活动次数显著减少(P<0.05),而其余时间点无显著差异;小鼠的平衡木实验评分和在疲劳转棒仪上停留的时间均无明显差异;小鼠的脏器指数、组织病理和血液生化指标也无显著变化。结论:鞍叶羊蹄甲以最大给药量(20 g/kg)灌胃小鼠基本没有急性毒性,安全性高,但要注意给药初期其对自主活动有一定抑制作用。

Objective:To study the acute toxicity of Bauhinia brachycarpa on mice.Methods:Maximum dose method was applied to the acute toxicity test.After intragastrical administration of B.brachycarpa to mice,successive observation was conducted in 14 days.During administration,the body weights of the mice was determined,and the behavioral performance of the mice was assessed by voluntary activity test,balance wood test and fatigue rotarod test.The 14th day after administration,the mice were sacrificed,the organ indexes and blood biochemical indexes were measured,and the histopathological changes were observed.Results:Compared with solvent control group,the weights of the mice in B.brachycarpa group did not significantly change;the voluntary activities of the mice obviously reduced in the 1^(st) day after administration,but had no significant changes at other observation times(P<0.05).There was no significant difference observed after the administration of B.brachycarpa in balance wood test and fatigue rotarod test.Nor there were any obvious variations in mice's organ indexes,histopathology and blood biochemical indexes after the administration.Conclusion:B.brachycarpa has almost no acute toxicity at the maximum dose(20 g/kg)by intragastric administration in mice which represents high safety,but its inhibitory effect on voluntary activities at the early stage of administration should be noticed.