藏区濒危药用植物独一味的叶绿体基因组特征

Chloroplast Genome Characteristics of a Tibetan Endangered Medicinal Plant Lamiophlomis rotata

目的:解析藏区药用植物独一味的叶绿体全基因组特征及确定其系统发育位置。方法:利用二代测序技术对独一味进行测序,经组装及注释,得到叶绿体全基因组序列;对其基因组特征进行解析,并结合唇形科近缘类群的叶绿体基因组序列,进行系统发育分析。结果:独一味叶绿体全基因组为典型的四分体结构,全长151808 bp,共注释了107个基因。基于贝叶斯法(BI)和最大似然法(ML)构建系统树的拓扑结构基本一致,独一味属与水苏属聚为一支。结论:独一味叶绿体全基因组特征首次得到解析,系统发育研究支持独一味属与水苏属近缘的传统分类处理,为独一味及其相关种的物种鉴定、资源保护及系统进化等研究奠定了基础。

Objective:To reveal the complete chloroplast genome characteristics of a medicinal plant in Tibetan region,namely Lamiophlomis rotate,and explore its phylogenetic position.Methods:In this study,the Next-Generation Sequencing technology was adopted to perform DNA sequencing for this species,and its complete chloroplast genome was obtained after assembly and annotation.Meanwhile,the genome characteristics were revealed,and its phylogeny was systematically analyzed,combined with the published chloroplast genomes of related species in Lamiaceae.Results:The complete chloroplast genome of L.rotata was a typical quadripartite structure,the total length of which was totally 151808 bp,and 107 genes were annotated.The topological structures between the Bayesian(BI)and the maximum likelihood(ML)were extremely similar,proving that Lamiophlomis and Stachys were gathered into one clade.Conclusion:The complete chloroplast genome characteristics of L.rotata were revealed for the first time,and its phylogenetic study supported the traditional treatment of regarding Lamiophlomis and Stachys as closely related genera.This study could provide scientific basis for studies such as species identification,resource conservation,and phyletic evolution of L.rotata and related species.