大学生人群中肥胖症与氧化应激相关性的初步研究

A Preliminary Study on the Correlation between Obesity and Oxidative Stress in College Students

目的:探讨大学生人群中肥胖症与氧化应激的相关性,为氧化炎症诊断标准的研制工作提供研究方案和技术。方法:随机选取大理大学在校学生80名作为研究对象,在体检时进行尿液采样;采用酶联免疫吸附试验(ELISA)对其尿液中8-羟基脱氧鸟苷(8-OHdG)含量进行测定,并根据身高、体质量数据计算体质指数(BMI);使用SPSS 17.0软件进行线性相关分析与单因素方差分析。结果:BMI值与尿液中8-OHdG含量呈正相关,其中r_(男生)=0.332、r_(女生)=0.338、r_(合计)=0.341;肥胖者和超重者尿液中8-OHdG含量均高于体质量正常者。结论:大学生肥胖症和氧化应激/氧化炎症作为能量摄入过剩的下游事件,二者之间虽然存在统计学相关性,但不存在因果关系,它们之间数量上存在的“镜像关系”,为利用肥胖症的诊断标准来探讨氧化炎症诊断标准提供了可能。

Objective:To explore the correlation between obesity and oxidative stress among college students,and to provide research programs and techniques for the development of diagnostic criteria for oxidative inflammation.Methods:80 students from Dali University were randomly selected as the subjects of study,and urine samples were taken during physical examination;the urine 8-hydroxy-2'-deoxyguanosine(8-OHdG)content was determined by enzyme linked immunosorbent assay(ELISA).And the body mass index(BMI)is calculated based on the height and body mass data;SPSS 17.0 software were used to perform linear correlation analysis and one-way analysis of variance.Results:BMI value was positively correlated with urine 8-OHdG content,among which r_(boys)=0.332,r_(girls)=0.338,and r_(total)=0.341;obese and overweight people had higher urine 8-OHdG levels than those of normal body mass.Conclusion:Obesity and oxidative stress/oxidative inflammation in college students are downstream events of excess energy intake.There is a statistical correlation between the two,but not causal relationship.There is a quantitative"mirror relationship"between them which provides the possibility to use the diagnostic criteria of obesity to explore the diagnostic criteria of oxidative inflammation.