孕早期外周血中lnc-C17orf64-1:1对子痫前期的预测价值

The predictive value of lnc-C17orf64-1:1 in peripheral blood in early pregnancy for preeclampsia

目的 探究孕早期孕妇外周血中lnc-C17orf64-1∶1的表达及其在子痫前期发病中的预测价值。方法 选择2018年9月至2019年9月于上海交通大学医学院附属国际和平妇幼保健院就诊分娩的孕妇,留取其孕早期(11~13周)外周血样本。根据妊娠结局是否为子痫前期分组:正常孕妇29例为对照组,子痫前期孕妇20例为子痫前期组。收集孕产妇相关临床资料,采用实时荧光定量PCR(qRT-PCR)检测孕早期外周血中lnc-C17orf64-1∶1的表达水平(ΔCT值),分析其与病情相关临床指标的相关性,并通过受试者工作特征(receiver operating characteristi, ROC)曲线分析其预测价值。结果 子痫前期组孕妇孕早期外周血中lnc-C17orf64-1∶1表达升高(ΔCT值降低);ROC曲线分析发现孕早期外周血lnc-C17orf64-1∶1预测子痫前期发病的曲线下面积(area under the curve, AUC)为0.9172(95%置信区间:0.8445-0.9899),敏感度80.00%,特异度86.21%,最大约登指数0.6621,最佳截断点13.71(ΔCT值),阳性预测值80.00%,阴性预测值86.21%;Spearman相关性分析发现孕早期外周血lnc-C17orf64-1∶1的ΔCT值与收缩压及舒张压均呈负相关。结论 孕早期外周血lnc-C17orf64-1∶1高表达与子痫前期密切相关,且对子痫前期早期诊断有一定预测价值。

Objective To explore the expression of lnc-C17 orf64-1∶1 in the peripheral blood of pregnant women in the first trimester and its predictive value in the pathogenesis of preeclampsia.Methods Peripheral blood samples in the first trimester(11~13 weeks) of the prospective cohort during pregnancy who visited and delivered at International Peace Maternity and Child Health Hospital Affiliated to Shanghai Jiaotong University School of Medicine from September 2018 to September 2019 were selected.According to whether the pregnancy outcome was preeclampsia or not: 29 normal pregnant women were the control group, and 20 preeclampsia pregnant women were the preeclampsia group.The relevant clinical data of pregnant and lying-in women were collected, and the expression level(ΔCT value) of lnc-C17 orf64-1∶1 in the peripheral blood of early pregnancy was detected by real-time fluorescence quantitative PCR(qRT-PCR),and its correlation with clinical indicators related to the disease was analyzed.Its predictive value was analyzed by receiver operating characteristic(ROC) curve.Results The expression of lnc-C17 orf64-1∶1 in the peripheral blood of pregnant women with preeclampsia increased in the first trimester(ΔCT value decreased);ROC curve analysis found that the area under the curve(AUC) of lnc-C17 orf64-1∶1 in the peripheral blood of the first trimester predicted the onset of preeclampsia was 0.9172(95% confidence interval: 0.8445-0.9899),sensitivity 80.00%,specificity 86.21%,maximum Youden index 0.6621,best cut-off point 13.71(ΔCT value),positive predictive value 80.00%,negative predictive value 86.21%;Spearman correlation analysis found that the ΔCT value of lnc-C17 orf64-1∶1 in peripheral blood in the first trimester was negatively correlated with both systolic and diastolic blood pressure.Conclusion The high expression of lnc-C17 orf64-1∶1 in peripheral blood in early pregnancy is closely related to preeclampsia, and has a certain predictive value for the early diagnosis of preeclampsia.