miR-146a在子痫前期患者中的表达及临床意义

Expression of miR-146a in patients with pre-eclampsia and their clinical significance

目的 探究miR-146a在正常产妇与子痫前期(preeclampsia, PE)患者胎盘组织中表达差异以及对子痫前期发病机制的影响。方法 选取临沂市中医医院2019年10月至2020年9月收治的PE患者24例作为PE组,另选取同期于本院分娩的24例健康妊娠妇女作为对照组。运用荧光实时定量聚合酶链反应(RT-PCR)测量其胎盘组织中miR-146a的表达,在线数据库TargetScan预测miR-146a作用靶点并运用蛋白质免疫印迹(western blot, WB)测定氧化低密度脂蛋白(oxidized low density lipoprotein, OXLDL)。并比较两组的基线资料及血压。结果 PE组胎盘组织中miR-146a水平高于对照组(P<0.05)。数据库预测到miR-146a与氧化低密度脂蛋白受体1(OLR1)有结合位点,WB结果表明PE组患者胎盘组织中OXLDL蛋白表达高于对照组,且定量分析结果表明两组比较,差异有统计学意义(P<0.05)。结论 miR-146a在PE患者胎盘组织中表达升高,这可能是作用于OXLDL受体抑制OXLDL阻断节点信号,这一发现可能是一种潜在的阻断节点信号通路的治疗策略。

Objective To explore the expression difference of miR-146 a in the placenta tissues of normal parturients and preeclampsia(PE) patients and its influence on the pathogenesis of preeclampsia.Methods A total of 24 PE patients who were admitted to Linyi Traditional Chinese Medicine Hospital from October 2019 to September 2020 were selected as PE group, and 24 healthy pregnant women who gave birth in this hospital during the same period were selected as the control group.Fluorescence real-time quantitative polymerase chain reaction(RT-PCR) was used to measure the expression of miR-146 a in placental tissues, the online database TargetScan was used to predict the target of miR-146 a and western blot(WB) was used to determine oxidized low-density lipoprotein(OXLDL).The baseline data and blood pressure of the two groups were compared.Results The level of miR-146 a in the placenta tissue of PE group was significantly higher than that of the control group(P<0.05).The database predicted that miR-146 a had a binding site with oxidized low-density lipoprotein receptor 1(OLR1).WB results indicated that the expression of OXLDL protein in the placenta tissue of the PE group was higher than that of the control group, and the quantitative analysis results showed that the difference between the two groups was significant(P<0.05).Conclusion The expression of miR-146 a is elevated in the placenta of PE patients.This may be due to the role of OXLDL receptors in inhibiting OXLDL from blocking nodal signaling.This finding may be a potential therapeutic strategy to block nodal signaling pathways.