布鲁氏菌病不同血清学检测方法结果分析

Analysis of Results of Different Serological Detections In Brucellosis

[目的]为了确定不同布病检测方法在临床应用中的意义。[方法]对疑似感染布鲁氏菌病羊血清同时作虎红平板凝集实验(RBT)、试管凝集实验(SAT)、ELISA抗体检测(cELISA)检测,分析它们的Kappa值、符合率、敏感度和特异性等。[结果]数据显示三者符合度较高,Kappa值在0.76以上,一致性相当可靠,其中RBT敏感度高,但假阳性率也高,相比SAT结果,cELISA敏感度和特异性都高,且两者Kappa值达到了0.85。[结论]综合判断临床工作中适合先用RBT广泛筛选,再用cELISA确诊的方法检测布鲁氏菌病。

[Objective]Determine the significance of different methods of brucellosis detection in clinical application.[Method]RBT,SAT,and cELISA were used to detect the suspected brucellosis-infected sheep serum.The Kappa value,coincidence rate,sensitivity,and specificity were analyzed.[Conclusion]Data showed that the three factors had high consistency,where the Kappa value was above 0.76,and the consistency was quite reliable.The sensitivity of RBT was high,but the false-positive rate was also high.Compared with the results of SAT,the sensitivity and specificity of cELISA were both high,and the Kappa value of RBT and cELISA were 0.85.[Conclusion]It is suitable to use RBT to screen widely in clinical practice,then use cELISA to confirm the diagnosis of brucellosis.