梅毒螺旋体黏附素蛋白TP0136不同亚型重组蛋白在神经梅毒患者血清诊断中的应用

Application of different subtypes of Treponema pallidum adhesin TP0136 recombinant proteins in serological diagnosis of patients with neurosyphilis

目的探讨不同亚型梅毒螺旋体(Tp)黏附素蛋白TP0136在神经梅毒(NS)患者血液中的诊断价值。方法比较60例NS患者与120例非神经梅毒的梅毒患者(NNS)一般情况。通过分子克隆技术构建表达和纯化Nichols Seattle TP0136(NST)和Nichols Houston TP0136(NHT)重组蛋白,采用ELISA方法检查NS和NNS患者血清中NST和NHT抗体水平。结果NS和NNS患者两组之间年龄、性别、来源地、职业无统计学差异(均P>0.05),而婚姻状况、神经系统症状、血清或血浆TRUST和TPPA滴度、血清或血浆TP-IgM阳性率、脑脊液TRUST和TPPA滴度均有统计学差异(均P<0.05)。NST在NS组和NNS组血清或血浆中抗体水平无差异(t=0.15,P=0.920);NHT在NNS组血清或血浆中抗体水平高于NS患者,差异有统计学意义(t=3.68,P=0.021)。结论Tp黏附素蛋白TP0136重组蛋白NHT或可作为NS诊断的潜在血液学标志物。

Objective To explore the diagnostic value of different subtypes of Treponema pallidum(Tp)adhesin protein TP0136 in the blood of neurosyphilitic patients.Methods The general conditions were compared in syphilitic patients with vs.without neuroinvolvement.Molecular cloning technique was used to express and purify Nichols Seattle TP0136(abbreviated as NST)and Nichols Houston TP0136(abbreviated as NHT)recombinant proteins.ELISA assay was used to measure serum levels of NST and NHT antibodies in neurosyphilitic and non-neurosyphilitic patients.Results There were no significant differences in age,gender,geographic region and occupation between neurosyphilitic and non-neurosyphilitic patients(P>0.05),while marital status,neurological symptoms,serum or plasma TRUST and TPPA titer,serum or plasma TP-IgM positive rate,cerebrospinal fluid TRUST and TPPA titer differed significantly(P<0.05);The antibody levels of NST in the serum or plasma of the neurosyphilitic and non-neurosyphilitic groups did not differ significantly(t=0.15,P=0.920).However,the antibody levels of NHT in the serum or plasma of the non-neurosyphilitic group were higher than that in neurosyphilitic patients(t=3.68,P=0.021).Conclusion Treponema pallidum adhesin protein TP0136 recombinant protein NHT can be used as a potential hematological marker for the diagnosis of neurosyphilis.