SARS-CoV-2病毒全基因组序列比对及进化分析

Sequence alignment and evolutionary analysis of SARS-CoV-2 virus

SARS-CoV-2病毒株是β属冠状病毒,它包括S、N、E、M 4个主要编码蛋白,是造成中国湖北省武汉市爆发重大公共卫生事件的罪魁祸首,目前对该病毒的系统性研究分析并不是很多.运用计算机辅助软件工具来对筛选自NCBI基因文库中的36条来自不同国家地区的SARS-CoV-2病毒株序列进行全序列比对分析,从而找出SARS-CoV-2病毒株可能存在的易变异区域,以及对所有序列的同源性及亲缘性进行分析,找出其传播径途及区域进化特点.通过对不同地区的SARS-CoV-2病毒株序列比对分析发现,SARS-CoV-2病毒株的ORF1ab蛋白编码区及S蛋白编码区存在高度变异性,为SARS-CoV-2病毒疫苗及相关药物的研制提供了相关参考.随后结合进化树和同源性进行分析发现,流行于美国等地区的SARS-CoV-2病毒与中国武汉发现的SARS-CoV-2病毒虽然有着相同的祖先,但其进化分支却是不同的,为SARS-CoV-2病毒的追根溯源提供了相关依据.

SARS-CoV-2 virus strain is a coronavirus of genusβ,including S,N,E and M,which is the main coding protein.It is the culprit that caused the major public health outbreak in Wuhan City,Hubei Province,China.Currently,there are not many systematic studies and analyses on this virus.This thesisused computer aided software tool for screening the NCBI gene library 36 from different countries and regions in the sequence of SARS-CoV-2 strains from the whole sequence alignment analysis,so as to find out the SARS-CoV-2 strains of possible variation and its propagation path way and regional evolution characteristicsthrough the analysis of all sequence homology and affinity.aSequence analysis of SARS-CoV-2 virus strains from different regions showed that the ORF1Ab protein coding region and the S protein coding region of SARS-CoV-2 virus strains were highly variable,which provided a relevant reference for the development of SARS-CoV-2 virus vaccine and related drugs.Based on the analysis of phylogenetic tree and homology,it was found that the SARS-CoV-2 virus prevalent in the United States and other regions had the same ancestor with the SARS-CoV-2 virus found in Wuhan,China,but their evolutionary branches were different,which provided a relevant basis for tracing the origin of SARS-CoV-2 virus.