电针百会穴对脾虚泄泻模型大鼠影响的研究

Study on the Effect of Electric Acupuncture at Baihui Acupoint on Rats with Spleen-deficiency Diarrhea

旨在探讨电针百会穴对脾虚泄泻大鼠的影响。选取64只Wistar大鼠,分成假手术组、模型组、百会组和阳性对照组。除假手术组外,其他各造模组以灌胃番泻叶浸液造模,待造模组出现脾虚泄泻症状后,百会组电针百会穴,阳性对照组灌胃蒙脱石散,持续2周。整个试验过程中观察并记录所有大鼠的症状体征、体质量和腹泻指数。试验结束后,检测各组大鼠血清中D-木糖、淀粉酶(amylase,AMS)和白细胞介素2(interleukin-2,IL-2)、IL^(-1)2、肿瘤坏死因子-α(tumor necrosis factor-α,TNF-α)含量,剖取脾和胰腺,并测定脾脏和胰腺指数。结果显示,模型组大鼠体型消瘦,神情呆滞,被毛杂乱,不愿走动,排水样稀便,稀便通常黏附于肛周及尾巴。电针百会穴可逐渐恢复大鼠的一般行为状态,显著提高雄性大鼠体质量(P<0.05);降低脾虚大鼠的腹泻指数(P<0.05);升高血清中D-木糖及AMS含量(P<0.05);降低血清IL-2、IL^(-1)2、TNF-α含量(P<0.05)。综上表明,电针百会穴可以改善脾虚泄泻大鼠的症状体征,升高体质量、调节脏器指数、提高消化吸收功能及缓解炎症情况,具有一定的治疗效果。能为临床治疗脾虚泄泻提供方便快捷的治疗手段,也可为研究百会穴的功效及其机制提供理论基础。

This study aims at exploring the effect of electric acupuncture at Baihui acupoint on rats with spleen-deficiency diarrhea.Sixty-four Wistar rats were selected and divided into four groups:sham operation group(Sham),model group(Model),electric acupuncture Baihui group(Baihui),positive control group(PC).Except for the Sham group,the rest groups were established spleen-deficiency diarrhea model by gavage of Folium Sennae decoction.The rats of Baihui group were electro-acupunctured at the Baihui acupoint and the rats of PC group were gavaged with montmorillonite powder for 2 weeks after successful modeling.During the whole experiment,the symptoms and signs,body mass and diarrhea index of all rats were observed and recorded.After the experiment,the serum levels of D-xylose,amylase(AMS),and inflammatory factors such as interleukin-2(IL-2),IL^(-1)2,and tumor necrosis factor-α(TNF-α)of all rats were detected.The spleen and pancreas were taken out,and the indexes of spleen and pancreas were measured.The results showed that the rats in the Model group suffered from weight loss,sluggish spirit,messy hair,decreased activity,watery stools,and perianal and tail adhering to loose stools.Electric acupuncture at Baihui acupoint could gradually restore the general behavioral state of rats with spleen-deficiency diarrhea,and significantly increase the body mass of male rats(P<0.05),decrease the diarrhea index(P<0.05),increase the content of serum D-xylose and amylase(P<0.05),decrease the serum content of IL-2,IL^(-1)2,and TNF-α(P<0.05).The results demonstrated that electric acupuncture the Baihui acupoint could improve the symptoms and signs of rats with spleen-deficiency diarrhea,increase body mass,adjust organ index,improve digestion and absorption,and relieve inflammation.Therefore,it has a certain therapeutic effect.This study provides a quick and convenient treatment method for clinical treatment of spleen-deficiency diarrhea,and could also provide a theoretical basis for the study of the efficacy and mechanism of Baihui acupoint.