免疫荧光检测绵羊体外受精胚胎Oct4、Cdx2表达研究

Study on Expression of Oct4 and Cdx2 in in vitro Fertilization Embryos of Sheep by Immunofluorescence Detection

Oct4基因和Cdx2基因是附植前胚胎发育的重要调控基因,并最终调控了胎儿和胎盘的发育,也在胚胎妊娠识别和附植时调控了干扰素(interferon tau,IFNT)的表达。本研究通过体外成熟、体外受精和体外培养获得了不同发育时期的绵羊胚胎,应用免疫荧光染色探讨了Oct4在早期胚胎的表达规律,结果表明:受精卵和早期卵裂胚中未检测到Oct4蛋白;16~32细胞期桑葚胚部分细胞表达Oct4蛋白;Oct4与Cdx2基因在绵羊囊胚滋养层细胞中存在共表达。

Oct4 and Cdx2 play important roles in the development of sheep pre-implantation embryos,which regulate the development of fetus and placenta.Oct4 and Cdx2 also regulate the expression of interferon-tau(IFNT),which plays a key role in embryo implantation and pregnancy recognization.In this study,embryos of different development stages were obtained by in vitro maturation,fertilization and cultured.Expression of Oct4 in the ovine embryos were analyzed using immunofluorescence staining.The results showed that expression of Oct4 protein was not detected in the zygotes and the early cleaved embryos.At the 16~32 morula cell stage,only a part of cells expressed Oct4 protein.Co-expression of Oct4 and Cdx2 was existed in the trophoblast of ovine blastocyst.