甲苯咪唑在团头鲂体内的物料平衡研究

Mass balance of mebendazole in body of blunt snout bream,Megalobrama amblycephala

为研究甲苯咪唑(mebendazole,MBZ)在团头鲂体内的分布和排泄规律,将MBZ以20 mg/kg单次灌服给药,在给药后的第0.25、0.5、1、2、3、4、5、6、7和8天进行水样采集,经固相萃取法提取净化后,采用液质联用仪(LC-MS/MS)测定水样中MBZ及其代谢物羟基甲苯咪唑、氨基甲苯咪唑的浓度。于第8天水样采完后,处死试验鱼,采集血液和肝、肾、鳃、肌肉等组织并称质量,用QuEChERS法进行提取净化,LC-MS/MS检测各组织中MBZ及其代谢物的浓度,并计算出各组织中的回收量。结果显示,MBZ及代谢物在心、肝、脾、鳃、肾、肠等组织中回收的量可达3332.26~5345.52μg,占总给药量的13.74%~23.74%。其中,在肠中的回收量最高,可达1248.29~2892.77μg,占总给药量的5.18%~12.85%。剩余组织中的回收量为987.22~1907.19μg,占总给药量的5.19%~11.40%。水中的回收量为1561.95~4623.15μg,占总给药量的6.44%~21.88%。总的回收量为6295.50~10983.41μg,总回收率为42.64%。以上结果显示,MBZ口服之后,主要分布在肠、肝和剩余组织,但是总回收率偏低。表明甲苯咪唑在团头鲂体内吸收较差,代谢路径主要通过粪尿进行排泄。

Mebendazole(MBZ)was administered to blunt snout breams(Megalobrama amblycephala)by oral gavage,and water samples were collected at corresponding time points of 0.25,0.5,1,2,3,4,5,6,7 and 8 d after drug administration to study the distribution and excretion of MBZ in the body of blunt snout bream.The liquid chromatography-mass spectrometry(LC-MS/MS)was used to determine the concentrations of MBZ and its metabolites in the water samples after extracted and purified by solid-phase extraction.The fish experimented were killed and its blood,liver,kidney,gill,muscle and other tissues were collected and weighed after the water samples were collected on the 8th day.QuEChERS method was used for extraction and purification,LC-MS/MS was used to detect the concentrations of MBZ and its metabolites in each tissue.The recovery amount in each tissue was calculated.Results showed that the recovery amount of MBZ and its metabolites in the heart,liver,spleen,gill,kidney,intestine etc.was ranged from3332.26 to 5345.52μg accounting for 13.74%-23.74%of the total dose,with the highest recovery amount in the intestine up to 5.18%-12.85%of the total dose.The recovered amount in other tissues was ranged from 987.22 to 1907.19μg accounting for 5.19%-11.40%of the total dose.The recovery amount in water was ranged from 1561.95 to 4623.15μg accounting for 6.44%-21.88%of the total dose.The total recovery amount of MBZ was ranged from 6295.50 to 10983.41μg,with the average recovery ratio of 42.64%.It is indicated that MBZ mainly exists in the form of mebendazole,hydroxybendazole and aminobendazole in the body of blunt snout breams,and no other metabolites were detected after oral gavage.MBZ and its metabolites were mainly distributed in the intestine,liver,other tissues,but the overall recovery ratio was low.It is indicated that mebendazole is poorly absorbed in the body of blunt snout breams,and its metabolic pathway is similar to other animals.Mebendazole is mainly excreted through feces and urine.