The origin of newborn hepatocytes in associating liver partition and portal vein ligation for staged hepatectomy(ALPPS)-derived regeneration

Associating liver partition and portal vein ligation for staged hepatectomy(ALPPS)has attracted interest in the field of hepatobiliary surgery worldwide(1).Given the significant improvement in morbidity and mortality,the ALPPS became safer in experienced centres,while research on the mechanism of ALPPS lags far behind clinical studies.Despite basic studies showing that activated hepatic stellates via Indian hedgehog/Gli1 pathway,mitochondria metabolism and inflammation were involved in ALPPS-derived regeneration,the cellular source of rapid self-duplication of hepatocytes during ALPPS stage I has not been well-documented(2,3).Therefore,the aim of this study was to explore the origin of newborn hepatocytes during ALPPS procedure.