舒敏中药提取物制备及其功效评价研究

Study on preparation and anti-allergy efficacy of Chinese herbal extracts

本文对香薷、山慈菇、独活、三棱、孩儿茶、远志六味中药进行提取,采用抑制透明质酸酶活性、清除自由基、抑制组胺释放实验来验证组方提取物的活性,并将其添加到乳液配方中,进行舒敏功效评价。结果表明:质量分数为6%的组方提取物对透明质酸酶抑制率达80.06%,对超氧阴离子自由基、羟自由基、DPPH自由基清除率均在70%以上;浓度为200μg/mL的组方提取物抑制组胺释放率达52%,这表明组方提取物对透明质酸酶、组胺释放及三种自由基均有一定的抑制或清除作用。人体功效评价结果表明,添加了组方提取物的乳液能够显著减少皮肤血红素(P<0.01),减少皮肤水分散失(P<0.01),提高皮肤水分含量(P<0.01),这表明该乳液能有效舒缓皮肤过敏症状,增强皮肤屏障功能。

In this paper,six kinds of traditional Chinese medicine:Herba Moslae,Pseudobulbus Cremastrae Seu Pleiones,Radix Angelicae Pubescentis,Rhizoma Sparganii,Catechu,Radix Polygalae were extracted,and the activity of the extract was verified by inhibiting hyaluronidase activity,scavenging free radicals,and inhibiting histamine release.And it was added to the emulsion formula to evaluate the efficacy of soothing.The results showed that the inhibition rate of 6%prescription extract on hyaluronidase was 80.06%,and the scavenging rates on Superoxide anion radical,Hydroxyl radical,and DPPH radical were more than 70%.The histamine release rate of 200μg/mL group extract was 52%,which indicated that the group extract had certain inhibition or scavenging effect on hyaluronidase,histamine release and three kinds of free radicals.The results of human efficacy evaluation showed that the emulsion with compound extract could significantly reduce the skin heme(P<0.01),reduce the skin water loss(P<0.01),and increase the skin water content(P<0.01).This shows that the emulsion can effectively relieve skin allergic symptoms and enhance skin barrier function.