RAD54L在肝细胞癌中的表达及临床意义

Expression of RAD54L and Its Clinical Significance in Hepatocellular Carcinoma

目的探讨RAD54L基因在肝细胞癌(HCC)中的诊断及预后价值。方法基于癌症基因组图谱(TCGA)的HCC数据,分析RAD54L对HCC的诊断价值以及与临床特征的关系。采用生存分析、单因素Cox回归、多因素Cox回归分析RAD54L在HCC患者中的预后价值。利用TIMER数据库分析RAD54L与免疫浸润细胞丰度的关系。利用基因集富集分析(GSEA)预测RAD54L高表达的相关通路。结果RAD54L基因在HCC中表达水平高于癌旁组织,且具有诊断价值,表达水平与性别、病理学分级等相关(P<0.05)。RAD54L表达水平是HCC独立的预后因素,高表达的患者预后较差。RAD54L表达水平与免疫浸润细胞的丰度相关。RAD54L高表达富集于细胞周期、同源重组等通路。结论RAD54L可能是HCC潜在的诊断和预后标志物。

Objective To investigate the value of RAD54L gene in the diagnosis and prognosis of hepatocellular carcinoma(HCC).Methods The diagnostic value of RAD54L and its relationship with clinical features were analyzed using data from The Cancer Genome Atlas(TCGA).Survival analysis,univariate Cox regression and multivariate Cox regression were used to indicate the prognostic value of RAD54L in HCC patients.The relationship between RAD54L and the abundance of immune infiltrating cells was analyzed using the TIMER database.Gene set enrichment analysis(GSEA)was used to analyze the pathway enrichment of RAD54L.Results Compared with the paraneoplastic tissues,RAD54L was significantly highly expressed in HCC,and it might be used to diagnose the disease.RAD54L was positively correlated with the gender and pathological grade of HCC patients(P<0.05).The expression of RAD54L was an independent risk factor in HCC,and the prognosis of patients with high expression was poor.The expression level of RAD54L was correlated with the abundance of immune infiltrating cells.RAD54L was highly expressed in cell cycle,homologous recombination pathways.Conclusion RAD54L may be a potential diagnostic and prognostic marker for HCC.