食品中金黄色葡萄球菌的快速检测及其评估

Determination of StaphlococcusAureus in Food and Evaluation of the Fast Kit

【目的】分析食品样品中金黄色葡萄球菌的含量,评估金黄色葡萄球菌快检试剂盒的可操作性和准确性。【方法】分别购买市售苹果脆片和真空包装水果玉米,采用国标GB 4789.10-2006和快检试剂盒测定其金黄色葡萄球菌含量,并以金黄色葡萄球菌为目标菌,采用人工污染样品,根据《食品快速检测方法评价技术规范》评估其快检试剂盒的相关指标。【结果】苹果脆片和水果玉米中金黄色葡萄球菌含量最高分别为20和50 CFU/g,均低于国标中相关食品中金黄色葡萄球菌限量要求。金黄色葡萄球菌快检试剂盒的灵敏度为93%,特异性为100%、假阴性率6.6%,假阳性率为0,显著性差异为0.5,相对准确度为96.7%,与参比方法检测数据差异不显著。【结论】金黄色葡萄球菌快检试剂盒检验食品中的金黄色葡萄球菌操作简单,检验结果较为准确,适合基层开展金黄色葡萄球菌的快速筛查。

【Objective】To analyze content of Staphylococcus aureus in food samples,and evaluate the accuracy and operability of the rapid kit for S.aureus.【Methods】Apple chips in bulk and fruit corn in vacuum packaging were sampled,and S.aureus was determined by the method in GB 4789,10-2006 and the rapid kit.S.aureus was taken as the target bacteria,artificial contamination treatments were used,and according to the Technical Specification for Evaluation of Rapid Food Testing Methods,the indexes of the rapid detection kit for S.aureus were evaluated.【Results】The highest content of S.aureus in samples of apple chips and fruit corn was 20 and 45 CFU/g,respectively,which were lower than the limit of pathogens in related foods.The sensitivity,specificity,false-negative rate,false-positive rate and relative accuracy of the kit were 93%,100%,6.6%,0,and 96.7%,respectively.Significant difference was not found between the rapid kit and the reference method.【Conclusion】The rapid kit is easy to operate and detect S.aureus accurately,which is suitable for fast checks.