血浆制备时间及速冻方法对冷沉淀质量的影响

Effect of Plasma Preparation Time and Quick Freezing Method on Cryoprecipitate Quality

目的:探讨血浆制备时间及速冻方法对冷沉淀质量的影响。方法:选取宁德市中心血站2020年6月至2020年12月期间采集的120例无偿献血者全血,按照随机数字表法分为A组与B组,各60例。制备时间段分别为6 h、8 h、12 h、18 h,每组不同制备时间段各15例。A组采用血浆速冻机速冻法,B组采用低温保存箱速冻法,比较两组冷沉淀在不同血浆制备时间段和速冻方法下的凝血因子Ⅷ(FⅧ)活性、纤维蛋白原含量。结果:A、B组组内不同制备时间下的FⅧ活性比较,差异均具有统计学意义(P<0.05);B组不同制备时间下的FⅧ活性均低于A组,差异均具有统计学意义(P<0.05)。A、B组组内不同制备时间下的纤维蛋白原含量比较,差异均无统计学意义(P>0.05);且两组不同制备时间下的纤维蛋白原含量,差异也均无统计学意义(P>0.05)。结论:不同的血浆制备时间对冷沉淀质量具有一定影响,血浆制备时间越长,冷沉淀凝血因子含量越低,采用血浆速冻机速冻法制备冷沉淀比低温保存箱速冻法的效果更好。

Objective To explore the influence of plasma preparation time and quick freezing method on cryoprecipitate quality.Methods 120 whole blood donors collected by Ningde Central Blood Station from June 2020 to December 2020 were selected.They were divided into two groups by random number table method including group A and group B,with 60 cases in each group.The preparation time was 6h,8h,12h and 18h,respectively,with 15 cases in each group at different preparation time.Group A was quick-frozen by plasma freezer,group B was quick-frozen by low-temperature storage box,and the coagulation factor VIII(FVIII)and fibrinogen content in two groups were compared under different plasma preparation time and different quick-frozen methods.Results There were statistically significant differences in FVIII activity between groups A and B under different preparation time(P<0.05);The FVIII activity of group B under different preparation time was lower than that of group A,with statistical significance(P<0.05).There was no statistical significance in the fibrinogen content of group A and group B under different preparation time(P>0.05);There was no significant difference in fibrinogen content between the two groups under different preparation time(P>0.05).Conclusion Different plasma preparation time has a certain effect on the quality of cryoprecipitate.The longer the plasma preparation time,the lower the content of cryoprecipitate coagulation factor.The effect of cryoprecipitate prepared by plasma quick-freezing method is better than that by low-temperature storage box.