人乳头状瘤病毒E6/E7诊断宫颈癌的应用价值

Application Value of Human Papillomavirus E6/E7 in the Diagnosis of Cervical Cancer

目的:探讨人乳头状瘤病毒(HPV)E6/E7诊断宫颈癌的应用价值。方法:选取2019年1月至2019年12月在揭阳市妇幼保健计划生育服务中心行宫颈病变筛查的受试者100例为研究对象。所有受试者均采用HPV脱氧核糖核酸(DNA)与HPV E6/E7信使核糖核酸(mRNA)进行检测,并以阴道镜组织病理学的诊断结果为金标准,比较两种检测方法的诊断效能,并比较不同年龄段下两种检测方法的诊断结果。结果:HPV E6/E7mRNA的诊断灵敏度、特异度、阳性预测值均高于HPV DNA,差异均具有统计学意义(P<0.05)。两种检测方法下,36~55岁患者的阳性检出率均高于其他年龄段,差异均具有统计学意义(P<0.05)。结论:采用HPV E6/E7 mRNA检测宫颈癌的灵敏度、特异度、阳性预测值均高于HPV DNA检测,临床诊断宫颈癌时,除了需考虑阴道镜组织检查、HPV E6/E7 mRNA及HPV DNA临床诊断结果外,还需结合患者年龄因素进行综合判断,避免漏诊。

Objective To explore the application value of human papillomavirus(HPV)E6/E7 in the diagnosis of cervical cancer.Methods A total of 100 subjects who underwent cervical lesion screening in Jieyang Maternal and Child Health Care Family Planning Service Center from January 2019 to December 2019 were selected as the research objects.All subjects were detected using HPV DNA and HPV E6/E7 messenger RNA(mRNA).The diagnostic efficacy of the two detection methods and the diagnostic results at different ages were compared using colposcopic histopathology as the gold standard and the diagnostic results of the two tests were compared at different ages..Results The diagnostic sensitivity,specificity and positive predictive value of HPV E6/E7 mRNA were higher than those of HPV DNA,and the differences were statistically significant(P<0.05).Under the two detection methods,the positive detection rates of patients aged 36-55 were higher than those of other age groups,with statistical significance(P<0.05).Conclusion The sensitivity,specificity and positive predictive value of HPV E6/E7 mRNA detection for cervical cancer are higher than that of HPV DNA detection.In addition to colposcopy tissue examination,HPV E6/E7 mRNA and HPV DNA clinical diagnosis results,a comprehensive judgment should also be made based on the patient's age to avoid missed diagnosis in the clinical diagnosis of cerviccal cancer..