摘要
目前新型冠状病毒肺炎(COVID-19)疫情仍在全球肆虐,但尚无针对该病毒的治疗特效药。在此背景,以美国化学文摘社(Chemical Abstracts Service, CAS)提供的SARS-CoV-2病毒及宿主蛋白靶标为研究对象,运用基因功能富集、蛋白网络等方法进行生物信息分析。结果发现,人网格蛋白介导型内吞和依赖型内吞是病毒进入宿主细胞的重要途径;病毒ORF6(Open Reading Frame, ORF)蛋白可影响细胞内核定位信号(Nuclear Localization Signal, NLS)介导蛋白入核的生物学过程。这些研究结果可为抗新型冠状病毒药物和疫苗的研发提供更多的可能性和思路。
COVID-19 epidemic is still raging around the world, and there is no effective specific treatment against the virus. In this paper, the SARS-CoV-2 viral and host cellular protein targets, which were provided by Chemical Abstracts Service(CAS), were analyzed through bioinformatics methods. GO enrichment and protein-protein network analysis methods were applied to explore biofunctions of these protein targets. Results showed that human clathrin-mediated endocytosis and dependent endocytosis were vital pathways for viruses entering host cells. In addition, viral ORF6 protein could influence the process that Nuclear Localization Signal(NLS) mediates the protein transferring into nucleus. These results may provide potential for the antiviral therapies and vaccines against SARS-CoV-2.
作者
赵子雨
刘畅
沈纪辰
李文硕
李欣
ZHAO Ziyu;LIU Chang;SHEN Jichen;LI Wenshuo;LI Xin(School of Medicine,Nankai University,Tianjin 300350,China;Biological Experimental Center,College of Life Science,Nankai University,Tianjin 300071,China)
出处
《生物信息学》
2022年第1期35-45,共11页
Chinese Journal of Bioinformatics
基金
天津市自然科学基金项目(No.18JCYBJC28600)
第十八届南开大学本科生创新科研“百项工程”项目(No.202010055851)。
关键词
新型冠状病毒
蛋白靶标
生信分析
SARS-CoV-2
Protein target
Bioinformatics analysis
